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Genetic manipulations have revealed the functions of RNA helicases in ribosomal RNA (rRNA) biogenesis in yeast. However, no report shows the role of an RNA helicase in rRNA formation in higher eukaryotes. This study reports the functional characterization of the frog homologue of nucleolar RNA helicase II/Gu (xGu or DDX21). Down-regulation of xGu in Xenopus laevis oocyte using an antisense oligodeoxynucleotide results in the depletion of 18 and 28 S rRNAs. The disappearance of 18 S rRNA is accompanied by an accumulation of 20 S, indicating that xGu is critical in the processing of 20 to 18 S rRNA. The degradation of 28 S rRNA into fragments smaller than 18 S is also associated with a specific decrease in the level of xGu protein. These effects are reversed in the presence of in vitro synthesized wild type xGu mRNA but not its helicase-deficient mutant form. Similar aberrant rRNA processing is observed when antibody against xGu is microinjected. The involvement of xGu in processing of rRNA is consistent with the localization of Gu protein to the granular and dense fibrillar components of PtK2 cell nucleoli by immunoelectron microscopy. Our results show that xGu is involved in the processing of 20 to 18 S rRNA and contributes to the stability of 28 S rRNA in Xenopus oocytes.

Titel:	Down-regulation of RNA helicase II/Gu results in the depletion of 18 and 28 S rRNAs in Xenopus oocyte.
Autor/in / Beteiligte Person:	Yang, H ; Zhou, J ; Ochs, RL ; Henning, D ; Jin, R ; Valdez, BC
Link:	Full Text Finder (Volltext)
Zeitschrift:	The Journal of biological chemistry, Jg. 278 (2003-10-03), Heft 40, S. 38847-59
Veröffentlichung:	2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology ; <i>Original Publication</i>: Baltimore, MD : American Society for Biochemistry and Molecular Biology, 2003
Medientyp:	academicJournal
ISSN:	0021-9258 (print)
DOI:	10.1074/jbc.M302258200
Schlagwort:	Amino Acid Sequence Animals Blotting, Northern Blotting, Western DNA, Complementary metabolism Electrophoresis, Polyacrylamide Gel Glutathione Transferase metabolism Kidney cytology Microscopy, Immunoelectron Models, Biological Molecular Sequence Data Nucleic Acid Conformation Oligonucleotides, Antisense chemistry Oligonucleotides, Antisense pharmacology Oocytes metabolism RNA metabolism RNA, Ribosomal metabolism Recombinant Fusion Proteins metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Time Factors Tissue Distribution Xenopus Xenopus laevis Down-Regulation RNA Helicases biosynthesis RNA Helicases genetics RNA, Ribosomal, 18S metabolism RNA, Ribosomal, 28S metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Language: English [J Biol Chem] 2003 Oct 03; Vol. 278 (40), pp. 38847-59. <i>Date of Electronic Publication: </i>2003 Jul 08. MeSH Terms: Down-Regulation* ; RNA Helicases / biosynthesis ; RNA Helicases / genetics ; RNA, Ribosomal, 18S / metabolism ; RNA, Ribosomal, 28S / metabolism ; Amino Acid Sequence ; Animals ; Blotting, Northern ; Blotting, Western ; DNA, Complementary / metabolism ; Electrophoresis, Polyacrylamide Gel ; Glutathione Transferase / metabolism ; Kidney / cytology ; Microscopy, Immunoelectron ; Models, Biological ; Molecular Sequence Data ; Nucleic Acid Conformation ; Oligonucleotides, Antisense / chemistry ; Oligonucleotides, Antisense / pharmacology ; Oocytes / metabolism ; RNA / metabolism ; RNA, Ribosomal / metabolism ; Recombinant Fusion Proteins / metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Time Factors ; Tissue Distribution ; Xenopus ; Xenopus laevis Grant Information: DK52341 United States DK NIDDK NIH HHS Substance Nomenclature: 0 (DNA, Complementary) ; 0 (Oligonucleotides, Antisense) ; 0 (RNA, Ribosomal) ; 0 (RNA, Ribosomal, 18S) ; 0 (RNA, Ribosomal, 28S) ; 0 (Recombinant Fusion Proteins) ; 63231-63-0 (RNA) ; EC 2.5.1.18 (Glutathione Transferase) ; EC 3.6.4.13 (RNA Helicases) Entry Date(s): Date Created: 20030710 Date Completed: 20031106 Latest Revision: 20210206 Update Code: 20240513

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Down-regulation of RNA helicase II/Gu results in the depletion of 18 and 28 S rRNAs in Xenopus oocyte.