Activation of the vrg6 promoter of Bordetella pertussis by RisA.
In: Journal of bacteriology, Jg. 187 (2005-03-01), Heft 5, S. 1648-58
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Zugriff:
The BvgAS two-component system positively regulates the expression of the virulence genes of Bordetella pertussis and negatively regulates a second set of genes whose function is unknown. The BvgAS-mediated regulation of the bvg-repressed genes is accomplished through the activation of expression of the negative regulator, BvgR. A second two-component regulatory system, RisAS, is required for expression of the bvg-repressed surface antigens VraA and VraB. We examined the roles of BvgR and RisA in the regulation of four bvg-repressed genes in B. pertussis. Our analyses demonstrated that all four genes are repressed by the product of the bvgR locus and are activated by the product of the risA locus. Deletion analysis of the vrg6 promoter identified the upstream and downstream boundaries of the promoter and, in contrast to previously published results, demonstrated that sequences downstream of the start of transcription are not required for the regulation of expression of vrg6. Gel mobility-shift experiments demonstrated sequence-specific binding of RisA to the vrg6 and vrg18 promoters, and led to the identification of two putative RisA binding sites. Finally, transcriptional analysis and Western blot analysis demonstrated that BvgR regulates neither the expression nor the stability of RisA.
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Activation of the vrg6 promoter of Bordetella pertussis by RisA.
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Autor/in / Beteiligte Person: | Cróinín, TO ; Grippe, VK ; Merkel, TJ |
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Zeitschrift: | Journal of bacteriology, Jg. 187 (2005-03-01), Heft 5, S. 1648-58 |
Veröffentlichung: | Washington, DC : American Society for Microbiology, 2005 |
Medientyp: | academicJournal |
ISSN: | 0021-9193 (print) |
DOI: | 10.1128/JB.187.5.1648-1658.2005 |
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