[Sequence analysis of integration sites of reticuloendotheliosis virus LTR in fowlpox vaccine virus genomes].
In: Wei sheng wu xue bao = Acta microbiologica Sinica, Jg. 46 (2006-08-01), Heft 4, S. 660-2
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Zugriff:
By use of genomic DNA prepared from 5 fowlpox virus (FPV) vaccines made in China (from Shandong, Beijing, Liaoning, Zhejing and Shanghai respectively) as the templates, reticuloendotheliosis virus (REV) LTR was amplified in PCR with a pair of primers synthesized according to the sequences flanking the integrated REV-5'LTR in FPV genomes published in US and Australia. Sequence analysis indicated that the REV-5'LTR integration sites in genomes of all 5 Chinese PFV vaccine products were identical to American and Australian FPV vaccines with integrated REV-5' LTR. Among the 5 FPV vaccine products made in China, 3 productes Vac-B-Ch, Vac-D-Ch and Vac-E-Ch had the integrated REV-5' LTR sequences of 223bp with 100% homology to that in American vaccine Vac-3-Am and Australian vaccine Vac-M3-Au. The integrated 505bp REV-LTR sequences in another Chinese products Vac-A-Ch and Vac-C-Ch had 99.6% homology to the integrated REV-LTR of American vaccine Vac-1-Am and Australian vaccine Vac-S-Au. However, REV-5'LTR integrated in all 5 FPV vaccines made in China gave only 75.4%-91.5% homology to LTR of a Chinese field strain HA9901 of REV. Based on the above results, it is reasonable to speculate that the virus stocks for the 5 Chinese FPV vaccine products were rather originally imported with their integrated REV LTR than recombinated with LTR of REV local field strains in China.
Titel: |
[Sequence analysis of integration sites of reticuloendotheliosis virus LTR in fowlpox vaccine virus genomes].
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Autor/in / Beteiligte Person: | Yu, LJ ; Cui, ZZ |
Zeitschrift: | Wei sheng wu xue bao = Acta microbiologica Sinica, Jg. 46 (2006-08-01), Heft 4, S. 660-2 |
Veröffentlichung: | Beijing : Science Press ; <i>Original Publication</i>: [Beijing, 1953?]-, 2006 |
Medientyp: | academicJournal |
ISSN: | 0001-6209 (print) |
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