Stereoselective determination of hydroxychloroquine and its metabolites in human urine by liquid-phase microextraction and CE.
In: Electrophoresis, Jg. 28 (2007-04-01), Heft 7, S. 1081-91
Online
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Zugriff:
Liquid-phase microextraction based on polypropylene hollow fibers and CE were applied for the chiral determination of hydroxychloroquine (HCQ) and its metabolites (desethylchloroquine, DCQ; desethylhydroxychloroquine, DHCQ; bisdesethylchloroquine, BDCQ) in human urine. The analytes were extracted from 3 mL of urine spiked with the internal standard (metoprolol) and alkalinized with 250 muL of 2 M NaOH. The analytes were extracted into 1-octanol impregnated in the pores of the hollow fiber, and into an acid acceptor solution inside the hollow fiber. The electrophoretic separations were carried out in 100 mmol/L Tris buffer (pH adjusted to 9.0 with phosphoric acid) containing 1% w/v S-beta-CD and 30 mg/mL HP-beta-CD with a constant voltage of +18 kV. The method was linear over the concentration range of 10-1000 ng/mL for each HCQ stereoisomer and 21-333 ng/mL for each metabolite stereoisomer. Within-day and between-day assay precision and accuracy for the analytes were studied at three concentration levels for each stereoisomer and were lower than 15%. The developed method was applied for the determination of the cumulative urinary excretion of HCQ, DCQ, and DHCQ after oral administration of rac-HCQ to a health volunteer. The results obtained are in agreement with previous literature data.
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Stereoselective determination of hydroxychloroquine and its metabolites in human urine by liquid-phase microextraction and CE.
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Autor/in / Beteiligte Person: | de Oliveira AR ; Cardoso, CD ; Bonato, PS |
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Zeitschrift: | Electrophoresis, Jg. 28 (2007-04-01), Heft 7, S. 1081-91 |
Veröffentlichung: | <Oct. 2004->: Weinheim : Wiley-VCH ; <i>Original Publication</i>: [Weinheim, Germany] : Verlag Chemie, [1980-, 2007 |
Medientyp: | academicJournal |
ISSN: | 0173-0835 (print) |
DOI: | 10.1002/elps.200600420 |
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