Analysis of Bordetella pertussis cya operon regulation by use of cya-lac fusions.
In: FEMS microbiology letters, Jg. 61 (1991-01-15), Heft 2-3, S. 251-6
Online
academicJournal
Zugriff:
In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neither is it activated by bvg, when introduced in trans. The cya-lac fusion is fully active when returned to B. pertussis by homologous recombination and responds to bvg-dependent activation and environmental regulation. These results indicate that in B. pertussis the activation of the cya operon by bvg is indirect.
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Analysis of Bordetella pertussis cya operon regulation by use of cya-lac fusions.
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Autor/in / Beteiligte Person: | Goyard, S ; Ullmann, A |
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Zeitschrift: | FEMS microbiology letters, Jg. 61 (1991-01-15), Heft 2-3, S. 251-6 |
Veröffentlichung: | 2015- : Oxford Oxford University Press ; <i>Original Publication</i>: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies., 1991 |
Medientyp: | academicJournal |
ISSN: | 0378-1097 (print) |
DOI: | 10.1016/0378-1097(91)90561-n |
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