Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation.

Cui, G ; Qi, Z ; et al.

In: Cell biology international, Jg. 33 (2009-03-01), Heft 3, S. 434-41

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Mouse embryonic fibroblasts (MEFs) have been used as feeder cells to support the growth of mouse embryonic stem cell (mESC) and primordial germ cells (PGC) in culture for many years. However, MEF preparation is a complex and tedious task. Recently, there are reports indicating that the microenvironment provided by bone marrow stromal cells could support the survival of embryonic-like stem cells in bone marrow. In this report, rat bone marrow derived mesenchymal progenitor cells (MPC) were used as feeder cells to culture mouse Oct4-GFP ES cell and ES cell derived germ cells. FACS results show that similar to MEF, rat MPC could efficiently support growth of the mouse Oct4-GFP ES cell line in culture (MPC 85.5 +/- 5.1% vs MEF 84.1 +/- 6.2%). ES cells could be subcultured for >15 passages without losing morphological characteristics. The cultured cells expressed stem cell marker alkaline phosphatase, Oct4, Sox2, and SSEA-1. Furthermore, rat MPC cells were able to support survival of germ cells isolated from mouse Oct4-GFP ES cell formed embryoid bodies (EB). After induction by retinoic acid for 7 days, some isolated cells differentiated to spermatogonial stem-like cells, expressing Mvh, Stra-8, Hsp90-a, integrinb1 and a6. Compared with traditional MEF culture systems, the rat MPC culture system is effective in supporting ES cell growth and is easy to prepare.

Titel:	Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation.
Autor/in / Beteiligte Person:	Cui, G ; Qi, Z ; Guo, X ; Qin, J ; Gui, Y ; Cai, Z
Link:	Full Text Finder (Volltext)
Zeitschrift:	Cell biology international, Jg. 33 (2009-03-01), Heft 3, S. 434-41
Veröffentlichung:	2013- : John Wiley & Sons Chichester : ; <i>Original Publication</i>: London, UK : Published for the International Federation for Cell Biology by Academic Press, c1993-, 2009
Medientyp:	academicJournal
ISSN:	1095-8355 (electronic)
DOI:	10.1016/j.cellbi.2009.01.008
Schlagwort:	Animals Bone Marrow Cells cytology Cell Differentiation Flow Cytometry Green Fluorescent Proteins biosynthesis Green Fluorescent Proteins metabolism Male Octamer Transcription Factor-3 biosynthesis Octamer Transcription Factor-3 metabolism Phenotype Rats Rats, Sprague-Dawley Recombinant Fusion Proteins biosynthesis Recombinant Fusion Proteins metabolism Cell Culture Techniques methods Embryonic Stem Cells cytology Germ Cells cytology Mesenchymal Stem Cells cytology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Cell Biol Int] 2009 Mar; Vol. 33 (3), pp. 434-41. MeSH Terms: Cell Culture Techniques / methods ; Embryonic Stem Cells / cytology ; Germ Cells / cytology ; Mesenchymal Stem Cells / cytology ; Animals ; Bone Marrow Cells / cytology ; Cell Differentiation ; Flow Cytometry ; Green Fluorescent Proteins / biosynthesis ; Green Fluorescent Proteins / metabolism ; Male ; Octamer Transcription Factor-3 / biosynthesis ; Octamer Transcription Factor-3 / metabolism ; Phenotype ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins / biosynthesis ; Recombinant Fusion Proteins / metabolism Substance Nomenclature: 0 (Octamer Transcription Factor-3) ; 0 (Pou5f1 protein, mouse) ; 0 (Recombinant Fusion Proteins) ; 147336-22-9 (Green Fluorescent Proteins) Entry Date(s): Date Created: 20090423 Date Completed: 20090713 Latest Revision: 20191111 Update Code: 20231215

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