High frequency of gross deletions in 5' LTR/gag and nef genes in patients infected with CRF02_AG of HIV type 1 who survived for over 20 years: an association with Korean red ginseng.
In: AIDS research and human retroviruses, Jg. 25 (2009-05-01), Heft 5, S. 535-41
academicJournal
Zugriff:
Abstract We have shown that Korean red ginseng (KRG) intake is associated with gross deletions in the 5' LTR/gag (gDeltaLTR/gag) and nef genes (gDeltanef) of patients infected with subtype B of HIV-1. Here, we investigated these effects in three long-term survivors (LTSs) of subtype CRF02_AG of HIV-1. The three LTSs were diagnosed with HIV in 1987, 1988, and 1989, and have been treated with KRG for 7-15 years. Thirty-two samples of peripheral blood mononuclear cells were obtained from the subjects and used to amplify the 5' LTR/gag and nef genes via nested PCR. We obtained 88 amplicons in 5' LTR/gag and 128 amplicons in nef. The frequency of gDeltaLTR/gag was significantly higher (37.5%) in three LTSs than in control patients (8.6%, p < 0.01). Eight amplicons (9.5%) contained premature stop codon(s) in the gDeltaLTR/gag in three LTSs. Fourteen of the 128 nef amplicons (10.9%) contained the gDeltanef, which was present in only two (7.7%) of the 26 amplicons from control subjects. Interestingly, gDeltanef was detected 7 years after the reinitiation of KRG intake in an LTS and, coincidently, CD4 T cell counts and CD4/CD8 ratios rapidly increased. These data indicate that long-term intake of KRG has the therapeutic potential to induce gross deletions in HIV-1.
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High frequency of gross deletions in 5' LTR/gag and nef genes in patients infected with CRF02_AG of HIV type 1 who survived for over 20 years: an association with Korean red ginseng.
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Autor/in / Beteiligte Person: | Cho, YK ; Jung, YS ; Sung, H ; Sim, MK ; Kim, YK |
Zeitschrift: | AIDS research and human retroviruses, Jg. 25 (2009-05-01), Heft 5, S. 535-41 |
Veröffentlichung: | Larchmont, NY : Mary Ann Liebert ; <i>Original Publication</i>: [New York] : Mary Ann Liebert, [c1987-, 2009 |
Medientyp: | academicJournal |
ISSN: | 1931-8405 (electronic) |
DOI: | 10.1089/aid.2008.0301 |
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