Two-step SSCF to convert AFEX-treated switchgrass to ethanol using commercial enzymes and Saccharomyces cerevisiae 424A(LNH-ST).

Jin, M ; Lau, MW ; et al.

In: Bioresource technology, Jg. 101 (2010-11-01), Heft 21, S. 8171-8

academicJournal

It is well known that simultaneous saccharification and co-fermentation (SSCF) reduces cellulosic ethanol production cost compared to separate hydrolysis and fermentation (SHF). However, the traditional SSCF process of converting Ammonia Fiber Expansion (AFEX) pretreated switchgrass to ethanol using both commercial enzymes and Saccharomyces cerevisiae 424A(LNH-ST) gave reduced ethanol yield due to lower xylose consumption. To overcome this problem we have developed a two-step SSCF process, in which xylan was hydrolyzed and fermented first followed by the hydrolysis and fermentation of glucan. Important parameters, such as temperature, cellulases loading during xylan hydrolysis and fermentation, initial OD(600) for inoculation of S. cerevisiae 424A(LNH-ST), and pH, were studied for best performance. Compared with traditional SSCF, the two-step SSCF showed higher xylose consumption and higher ethanol yield. The sugar conversion was also enhanced from 70% by enzymatic hydrolysis to 82% by two-step SSCF. One important finding is that the residue from enzymatic hydrolysis plays a significant role in reducing xylose consumption and ethanol metabolic yield during SSCF.

Titel:	Two-step SSCF to convert AFEX-treated switchgrass to ethanol using commercial enzymes and Saccharomyces cerevisiae 424A(LNH-ST).
Autor/in / Beteiligte Person:	Jin, M ; Lau, MW ; Balan, V ; Dale, BE
Zeitschrift:	Bioresource technology, Jg. 101 (2010-11-01), Heft 21, S. 8171-8
Veröffentlichung:	Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-, 2010
Medientyp:	academicJournal
ISSN:	1873-2976 (electronic)
DOI:	10.1016/j.biortech.2010.06.026
Schlagwort:	Carbohydrate Metabolism Hydrogen-Ion Concentration Hydrolysis Polysorbates metabolism Saccharomyces cerevisiae enzymology Temperature Xylose metabolism Ammonia metabolism Biotechnology methods Cellulase metabolism Ethanol metabolism Fermentation physiology Poaceae metabolism Saccharomyces cerevisiae metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Language: English [Bioresour Technol] 2010 Nov; Vol. 101 (21), pp. 8171-8. <i>Date of Electronic Publication: </i>2010 Jun 30. MeSH Terms: Ammonia / metabolism ; Biotechnology / methods ; Cellulase / metabolism ; Ethanol / metabolism ; Fermentation / physiology ; Poaceae / metabolism ; Saccharomyces cerevisiae / *metabolism ; Carbohydrate Metabolism ; Hydrogen-Ion Concentration ; Hydrolysis ; Polysorbates / metabolism ; Saccharomyces cerevisiae / enzymology ; Temperature ; Xylose / metabolism Substance Nomenclature: 0 (Polysorbates) ; 3K9958V90M (Ethanol) ; 7664-41-7 (Ammonia) ; A1TA934AKO (Xylose) ; EC 3.2.1.4 (Cellulase) Entry Date(s): Date Created: 20100629 Date Completed: 20101021 Latest Revision: 20220317 Update Code: 20240513

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