Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific primers derived by suppressive subtractive hybridization.
In: Applied and environmental microbiology, Jg. 77 (2011-06-01), Heft 11, S. 3898-902
Online
academicJournal
Zugriff:
A quantitative PCR (qPCR) assay targeting a gene identified by suppressive subtractive hybridization (SSH) was developed to detect Propionibacterium acidipropionici P169, with a threshold of 10(4) CFU/U of dairy feed or rumen fluid. The report is the first using a molecular marker generated by SSH to quantify a bacterial strain in environmental samples.
Titel: |
Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific primers derived by suppressive subtractive hybridization.
|
---|---|
Autor/in / Beteiligte Person: | Peng, M ; Smith, AH ; Rehberger, TG |
Link: | |
Zeitschrift: | Applied and environmental microbiology, Jg. 77 (2011-06-01), Heft 11, S. 3898-902 |
Veröffentlichung: | Washington, American Society for Microbiology., 2011 |
Medientyp: | academicJournal |
ISSN: | 1098-5336 (electronic) |
DOI: | 10.1128/AEM.02586-10 |
Schlagwort: |
|
Sonstiges: |
|