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Objective: To assess the effect of caspase 3 inhibition, in the expression of intracellular antigens induced by apoptosis.

Material and Methods: Skin explants of neonatal Balb/c mice were used to assess the autoantigen expression. Skin was obtained by punch biopsies, tissues were cultured in DMEM; cell death was induced by chemicals and assessed by TUNEL. The expression of La, Ro, Sm, RNP, Cajal Bodies and NuMa antigens were monitored by immunohistochemistry using autoantibodies or monoclonal antibodies against these antigens.

Results: Chemicals used to induce cell death, successfully produced apoptosis or necrosis in more than 60% of keratinocytes, and viability was significantly decreased when it was compared with those in controls. An increased expression of all skin intracellular antigens in skin biopsies treated with chemicals, major antigenic expression was detected with anti-La and anti-Ro antibodies. The caspase 3 inhibitor DEVD-CMK significantly decreased the expression of antigens induced by chemicals.

Conclusion: By this result we can infer that caspase inhibitors modify apoptosis and decrease the autoantigens associated to cell death.

Titel:	Caspase 3 blocking avoids the expression of autoantigens triggered by apoptosis in neonatal Balb/c mice skin.
Autor/in / Beteiligte Person:	Ramírez-Sandoval, R ; Vazquez-del-Mercado, M ; Daneri-Navarro, A ; Bollain-y-Goytia, JJ ; Avalos-Diaz, E ; Herrera-Esparza, R
Zeitschrift:	Reumatismo, Jg. 63 (2011-03-01), Heft 1, S. 29-37
Veröffentlichung:	Pavia, Italy : PagePress ; <i>Original Publication</i>: Milan : Longanesi, 2011
Medientyp:	academicJournal
ISSN:	0048-7449 (print)
DOI:	10.4081/reumatismo.2011.29
Schlagwort:	Animals Animals, Newborn Autoimmune Diseases etiology Biopsy Camptothecin pharmacology Cells, Cultured drug effects Cells, Cultured enzymology Cells, Cultured immunology Cycloheximide pharmacology Cysteine Proteinase Inhibitors pharmacology Drug Evaluation, Preclinical Hydrogen Peroxide pharmacology In Situ Nick-End Labeling Mercuric Chloride pharmacology Mice Mice, Inbred BALB C Organ Culture Techniques Skin enzymology Amino Acid Chloromethyl Ketones pharmacology Apoptosis immunology Autoantigens biosynthesis Autoimmune Diseases prevention & control Caspase Inhibitors Cysteine Proteinase Inhibitors therapeutic use Skin immunology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Language: English [Reumatismo] 2011 Mar; Vol. 63 (1), pp. 29-37. MeSH Terms: Caspase Inhibitors* ; Amino Acid Chloromethyl Ketones / pharmacology ; Apoptosis / immunology ; Autoantigens / biosynthesis ; Autoimmune Diseases / prevention & control ; Cysteine Proteinase Inhibitors / therapeutic use ; Skin / immunology ; Animals ; Animals, Newborn ; Autoimmune Diseases / etiology ; Biopsy ; Camptothecin / pharmacology ; Cells, Cultured / drug effects ; Cells, Cultured / enzymology ; Cells, Cultured / immunology ; Cycloheximide / pharmacology ; Cysteine Proteinase Inhibitors / pharmacology ; Drug Evaluation, Preclinical ; Hydrogen Peroxide / pharmacology ; In Situ Nick-End Labeling ; Mercuric Chloride / pharmacology ; Mice ; Mice, Inbred BALB C ; Organ Culture Techniques ; Skin / enzymology Substance Nomenclature: 0 (Amino Acid Chloromethyl Ketones) ; 0 (Autoantigens) ; 0 (Caspase Inhibitors) ; 0 (Cysteine Proteinase Inhibitors) ; 0 (benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone) ; 53GH7MZT1R (Mercuric Chloride) ; 98600C0908 (Cycloheximide) ; BBX060AN9V (Hydrogen Peroxide) ; XT3Z54Z28A (Camptothecin) Entry Date(s): Date Created: 20110422 Date Completed: 20110915 Latest Revision: 20191112 Update Code: 20240513

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Caspase 3 blocking avoids the expression of autoantigens triggered by apoptosis in neonatal Balb/c mice skin.