A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.
In: Science (New York, N.Y.), Jg. 332 (2011-06-10), Heft 6035, S. 1313-7
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Zugriff:
The DNA damage response (DDR) is brought about by a protein kinase cascade that orchestrates DNA repair through transcriptional and posttranslational mechanisms. Cell cycle arrest is a hallmark of the DDR. We screened for cells that lacked damage-induced cell cycle arrest and uncovered a critical role for Fanconi anemia and homologous recombination proteins in ATR (ataxia telangiectasia and Rad3-related) signaling. Three DDR candidates, the RNA processing protein INTS7, the circadian transcription factor CLOCK, and a previously uncharacterized protein RHINO, were recruited to sites of DNA damage. RHINO independently bound the Rad9-Rad1-Hus1 complex (9-1-1) and the ATR activator TopBP1. RHINO was recruited to sites of DNA damage by the 9-1-1 complex to promote Chk1 activation. We suggest that RHINO functions together with the 9-1-1 complex and TopBP1 to fully activate ATR.
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A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.
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Autor/in / Beteiligte Person: | Cotta-Ramusino, C ; McDonald ER 3rd ; Hurov, K ; Sowa, ME ; Harper, JW ; Elledge, SJ |
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Zeitschrift: | Science (New York, N.Y.), Jg. 332 (2011-06-10), Heft 6035, S. 1313-7 |
Veröffentlichung: | <Oct. 4, 1991- > : Washington, DC : American Association for the Advancement of Science ; <i>Original Publication</i>: New York, N.Y. : [s.n.] 1880-, 2011 |
Medientyp: | academicJournal |
ISSN: | 1095-9203 (electronic) |
DOI: | 10.1126/science.1203430 |
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