Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence.
In: Meat science, Jg. 59 (2001-10-01), Heft 2, S. 165-8
academicJournal
Zugriff:
A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm(2) pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 10(4) cfu/cm(2).
| Titel: |
Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence.
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| Autor/in / Beteiligte Person: | Werlein, HD |
| Link: | |
| Zeitschrift: | Meat science, Jg. 59 (2001-10-01), Heft 2, S. 165-8 |
| Veröffentlichung: | <200?>- : Oxford : Elsevier ; <i>Original Publication</i>: Barking, Essex, Eng., Applied Science Publishers, 2001 |
| Medientyp: | academicJournal |
| ISSN: | 0309-1740 (print) |
| DOI: | 10.1016/s0309-1740(01)00066-3 |
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