Detection of the hybrid BCR/ABL messenger RNA in single CFU-GM colonies using the polymerase chain reaction.
In: Experimental hematology, Jg. 18 (1990-11-01), Heft 10, S. 1142-4
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Zugriff:
In order to study which hemopoietic precursor cells express the hybrid BCR/ABL fusion mRNA we have developed a technique based on the polymerase chain reaction (PCR) for the examination of single hemopoietic colonies grown on semi-solid agar. The technique was developed by examining single CFU-GM colonies grown from newly diagnosed patients with chronic myeloid leukaemia (CML). RNA was isolated from individual 14 day colonies and reverse transcribed to a complementary DNA (cDNA) copy which formed the substrate for a PCR. We have studied 3 cases of CML using this method and have found that 5 out of 5, 9 out of 10 and 20 out of 23 colonies examined were positive. Thus we describe a simple and useful technique for the study of gene expression in a limited number of hemopoietic precursor cells.
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Detection of the hybrid BCR/ABL messenger RNA in single CFU-GM colonies using the polymerase chain reaction.
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Autor/in / Beteiligte Person: | Hernandez, A ; Osterholz, J ; Price, CM ; Wiedemann, LM ; Gordon, MY ; Goldman, JM ; Morgan, GJ |
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Zeitschrift: | Experimental hematology, Jg. 18 (1990-11-01), Heft 10, S. 1142-4 |
Veröffentlichung: | 1999- : Amsterdam : Elsevier Science Inc. ; <i>Original Publication</i>: Copenhagen, Munksgaard., 1990 |
Medientyp: | academicJournal |
ISSN: | 0301-472X (print) |
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