Depletion of mitochondrial fission factor DRP1 causes increased apoptosis in human colon cancer cells.

Inoue-Yamauchi, A ; Oda, H

In: Biochemical and biophysical research communications, Jg. 421 (2012-04-27), Heft 1, S. 81-5

academicJournal

Mitochondria play a critical role in regulation of apoptosis, a form of programmed cell death, by releasing apoptogenic factors including cytochrome c. Growing evidence suggests that dynamic changes in mitochondrial morphology are involved in cellular apoptotic response. However, whether DRP1-mediated mitochondrial fission is required for induction of apoptosis remains speculative. Here, we show that siRNA-mediated DRP1 knockdown promoted accumulation of elongated mitochondria in HCT116 and SW480 human colon cancer cells. Surprisingly, DRP1 down-regulation led to decreased proliferation and increased apoptosis of these cells. A higher rate of cytochrome c release and reductions in mitochondrial membrane potential were also revealed in DRP1-depleted cells. Taken together, our present findings suggest that mitochondrial fission factor DRP1 inhibits colon cancer cell apoptosis through the regulation of cytochrome c release and mitochondrial membrane integrity.

Titel:	Depletion of mitochondrial fission factor DRP1 causes increased apoptosis in human colon cancer cells.
Autor/in / Beteiligte Person:	Inoue-Yamauchi, A ; Oda, H
Zeitschrift:	Biochemical and biophysical research communications, Jg. 421 (2012-04-27), Heft 1, S. 81-5
Veröffentlichung:	<2002- >: San Diego, CA : Elsevier ; <i>Original Publication</i>: New York, Academic Press., 2012
Medientyp:	academicJournal
ISSN:	1090-2104 (electronic)
DOI:	10.1016/j.bbrc.2012.03.118
Schlagwort:	Colonic Neoplasms metabolism Cytochromes c metabolism Dynamins GTP Phosphohydrolases genetics Gene Knockdown Techniques HCT116 Cells Humans Membrane Proteins genetics Microtubule-Associated Proteins genetics Mitochondrial Membranes enzymology Mitochondrial Membranes ultrastructure Mitochondrial Proteins genetics RNA, Small Interfering genetics Apoptosis genetics Colonic Neoplasms pathology GTP Phosphohydrolases metabolism Membrane Proteins metabolism Microtubule-Associated Proteins metabolism Mitochondrial Proteins metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biochem Biophys Res Commun] 2012 Apr 27; Vol. 421 (1), pp. 81-5. <i>Date of Electronic Publication: </i>2012 Apr 02. MeSH Terms: Apoptosis / genetics ; Colonic Neoplasms / pathology ; GTP Phosphohydrolases / metabolism ; Membrane Proteins / metabolism ; Microtubule-Associated Proteins / metabolism ; Mitochondrial Proteins / metabolism ; Colonic Neoplasms / metabolism ; Cytochromes c / metabolism ; Dynamins ; GTP Phosphohydrolases / genetics ; Gene Knockdown Techniques ; HCT116 Cells ; Humans ; Membrane Proteins / genetics ; Microtubule-Associated Proteins / genetics ; Mitochondrial Membranes / enzymology ; Mitochondrial Membranes / ultrastructure ; Mitochondrial Proteins / genetics ; RNA, Small Interfering / genetics Substance Nomenclature: 0 (Membrane Proteins) ; 0 (Mff protein, human) ; 0 (Microtubule-Associated Proteins) ; 0 (Mitochondrial Proteins) ; 0 (RNA, Small Interfering) ; 9007-43-6 (Cytochromes c) ; EC 3.6.1.- (GTP Phosphohydrolases) ; EC 3.6.5.5 (DNM1L protein, human) ; EC 3.6.5.5 (Dynamins) Entry Date(s): Date Created: 20120411 Date Completed: 20120622 Latest Revision: 20201209 Update Code: 20240513

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