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Objective: The aim of this study was to determine whether the mobilization and recruitment of endothelial progenitor cells (EPCs) contribute to the protection of kidneys from ischemia/reperfusion (I/R) injury after ischemic preconditioning (IPC) during the late phase.

Methods: Seventy-five male Sprague-Dawley rats were divided into the following groups: sham-operated (group A; n = 25), ischemia/reperfusion hosts that underwent 45 minutes of left renal artery ischemia (group B; n = 25), and ischemic preconditioning-treated group (group C; n = 25). Group C underwent 3 cycles of 5 minutes of occlusion and 5 minutes of reperfusion followed by 24 hours of reperfusion before the following 45 minutes of occlusion. Serum samples were collected and renal tissues harvested for histological examination terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, immunohistochemical staining, and Western blot analysis to determine the expression levels of CD34, VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2)/flk-1, vascular endothelial growth factor (VEGF), and stromal cell-derived factor-1α (SDF-1α).

Results: Compared with group B, the levels of blood urea nitrogen (BUN), serum creatinine (Scr) and acute tubulointerstitial injury at 24 hours after operation were significantly reduced in group C. At 72 hours, tubular epithelial cell apoptosis was also decreased (17.6 ± 4.45 vs 63.8 ± 6.10; P < .01). CD34+ and flk-1+ cells that mostly accumulated in the medullopapillary parenchyma were significantly increased at 72 hours (P < .05). Expression levels of VEGF and SDF-1α were also significantly higher in group C (P < .05).

Conclusion: The present work suggested that IPC protected kidneys from IR injury in the later phase through enhanced mobilization and recruitment of EPCs. VEGF and SDF-1α may play important roles in this protective effect.

Titel:	Effects of ischemic preconditioning in the late phase on homing of endothelial progenitor cells in renal ischemia/reperfusion injury.
Autor/in / Beteiligte Person:	Bo, CJ ; Chen, B ; Jia, RP ; Zhu, JG ; Cao, P ; Liu, H ; Wu, R ; Ge, YZ ; Wu, JP
Zeitschrift:	Transplantation proceedings, Jg. 45 (2013-03-01), Heft 2, S. 511-6
Veröffentlichung:	New York, N.Y. : Elsevier Science Inc. ; <i>Original Publication</i>: New York Stratton., 2013
Medientyp:	academicJournal
ISSN:	1873-2623 (electronic)
DOI:	10.1016/j.transproceed.2012.05.095
Schlagwort:	Animals Antigens, CD34 metabolism Apoptosis Biomarkers metabolism Blood Urea Nitrogen Blotting, Western Chemokine CXCL12 metabolism Creatinine blood Disease Models, Animal Immunohistochemistry In Situ Nick-End Labeling Kidney pathology Kidney physiopathology Male Rats Rats, Sprague-Dawley Reperfusion Injury pathology Reperfusion Injury physiopathology Time Factors Vascular Endothelial Growth Factor A metabolism Vascular Endothelial Growth Factor Receptor-2 metabolism Cell Movement Endothelial Cells pathology Ischemic Preconditioning Kidney blood supply Reperfusion Injury prevention & control Stem Cells pathology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Transplant Proc] 2013 Mar; Vol. 45 (2), pp. 511-6. MeSH Terms: Cell Movement* ; Ischemic Preconditioning* ; Endothelial Cells / pathology ; Kidney / blood supply ; Reperfusion Injury / prevention & control ; Stem Cells / pathology ; Animals ; Antigens, CD34 / metabolism ; Apoptosis ; Biomarkers / metabolism ; Blood Urea Nitrogen ; Blotting, Western ; Chemokine CXCL12 / metabolism ; Creatinine / blood ; Disease Models, Animal ; Immunohistochemistry ; In Situ Nick-End Labeling ; Kidney / pathology ; Kidney / physiopathology ; Male ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury / pathology ; Reperfusion Injury / physiopathology ; Time Factors ; Vascular Endothelial Growth Factor A / metabolism ; Vascular Endothelial Growth Factor Receptor-2 / metabolism Substance Nomenclature: 0 (Antigens, CD34) ; 0 (Biomarkers) ; 0 (Chemokine CXCL12) ; 0 (Vascular Endothelial Growth Factor A) ; 0 (vascular endothelial growth factor A, rat) ; AYI8EX34EU (Creatinine) ; EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2) Entry Date(s): Date Created: 20130319 Date Completed: 20130830 Latest Revision: 20151119 Update Code: 20231215

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Effects of ischemic preconditioning in the late phase on homing of endothelial progenitor cells in renal ischemia/reperfusion injury.