Association of cytokines, high sensitive C-reactive protein, VEGF and beta-defensin-1 gene polymorphisms and their protein expressions with chronic periodontitis in the Chinese population.
In: The International journal of biological markers, Jg. 28 (2013-04-23), Heft 1, S. 100-7
academicJournal
Zugriff:
Purposes: This study aimed at investigating the association between interleukin-6 (IL-6), interleukin-12 (IL-12), C-reactive protein (CRP), vascular endothelial growth factor (VEGF) and β-defensin-1 polymorphisms and the susceptibility to periodontitis in the Chinese population.
Methods: DNA was extracted from the blood samples of 532 healthy individuals and 122 chronic periodontitis (CP) patients enrolled in the study. The genes encoding IL-6, IL-12, CRP, VEGF and β-defensin-1 were amplified using PCR and digested with restriction enzymes. The protein expression of the abovementioned genes was determined by ELISA. Differences in the allele/genotype frequencies were assessed with the chi-square test.
Results: The frequencies of the C/C genotypes of IL-6, IL-12, and VEGF were higher in CP patients than healthy controls (66.3% vs 25.9%; 27.8% vs 19.9%; and 64.8% vs 52.1%, respectively). In the patients' group we also recorded frequencies of the A/A genotypes of CRP and VEGF higher than in healthy controls (63.1% vs 58.1% and 64.8% vs 35.2%, respectively). Protein production evaluated by ELISA demonstrated significant differences between CP patients and healthy controls for IL-6, IL-12, CRP, VEGF and β-defensin-1.
Conclusions: The genotypes of IL-6, IL-12, VEGF and β-defensin-1 and their protein productions were associated with CP in a Chinese population. Genotypes and serum levels of CRP were associated with CP, but alleles frequency showed no difference between CP patients and healthy controls.
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Association of cytokines, high sensitive C-reactive protein, VEGF and beta-defensin-1 gene polymorphisms and their protein expressions with chronic periodontitis in the Chinese population.
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Autor/in / Beteiligte Person: | Tian, Y ; Li, JL ; Hao, L ; Yue, Y ; Wang, M ; Loo, WT ; Cheung, MN ; Chow, LW ; Liu, Q ; Yip, AY ; Ng, EL ; Chow, CY |
Zeitschrift: | The International journal of biological markers, Jg. 28 (2013-04-23), Heft 1, S. 100-7 |
Veröffentlichung: | 2018- : Thousand Oaks, CA : SAGE Publishing ; <i>Original Publication</i>: Milano : Wichtig editore, c1986-, 2013 |
Medientyp: | academicJournal |
ISSN: | 1724-6008 (electronic) |
DOI: | 10.5301/jbm.5000010 |
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