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Aim: This study evaluated the cytotoxicity of a dental bonding model resin (DBMR) submitted to different photo-activation distances.

Methods: A monomer mixture based on Bis-GMA and HEMA was used to assess the cytotoxicity in a mouse fibroblast-cell line. To promote different photo-activation distances glass slides were interposed between DBMR surface and halogen light curing unit (LCU) tip. Afterwards, the specimens were immersed in RPMI culture medium for 24 h to obtain extracts. The extracts were incubated in contact with the cells for 24 h. Finally, an MTT colorimetric assay was used to assess the cytotoxicity. The cell viability data (absorbance) were analyzed by one way ANOVA followed by Tukey's test (P<0.05).

Results: The light output decreased according to the increase in the number of glass slides between the halogen LCU tip and DBMR surface. Yet, the distance between the tip of the curing light system and the specimens had significant influence on the cytotoxicity. All extracts produced by groups submitted to different photo-activation distances showed cytotoxic effect after 24h of incubation.

Conclusion: The photo-activation distance and the interposition of glass slides between LCU tip and DBMR was shown to play an important role in the cytotoxic effect.

Titel:	Influence of different photo-activation distances on cytotoxicity of a dental adhesive model resin.
Autor/in / Beteiligte Person:	De Carvalho, RV ; Fernández, MR ; Poli-Frederico, RC ; Guiraldo, RD ; Lopes, MB ; Berger, SB ; Moura, SK ; Demarco, FF
Zeitschrift:	Minerva stomatologica, Jg. 62 (2013-06-01), Heft 6, S. 199-205
Veröffentlichung:	Torino : Edizioni Minerva Medica, 2013
Medientyp:	academicJournal
ISSN:	0026-4970 (print)
Schlagwort:	Animals Bisphenol A-Glycidyl Methacrylate radiation effects Camphor analogs & derivatives Camphor pharmacology Cell Survival Halogens Hydrophobic and Hydrophilic Interactions Materials Testing Methacrylates radiation effects Mice Photochemistry Photoinitiators, Dental pharmacology Resins, Synthetic radiation effects para-Aminobenzoates pharmacology Bisphenol A-Glycidyl Methacrylate toxicity Curing Lights, Dental Light-Curing of Dental Adhesives methods Methacrylates toxicity NIH 3T3 Cells drug effects Resins, Synthetic toxicity
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Comparative Study; Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't Language: English [Minerva Stomatol] 2013 Jun; Vol. 62 (6), pp. 199-205. MeSH Terms: Curing Lights, Dental* ; Bisphenol A-Glycidyl Methacrylate / toxicity ; Light-Curing of Dental Adhesives / methods ; Methacrylates / toxicity ; NIH 3T3 Cells / drug effects ; Resins, Synthetic / *toxicity ; Animals ; Bisphenol A-Glycidyl Methacrylate / radiation effects ; Camphor / analogs & derivatives ; Camphor / pharmacology ; Cell Survival ; Halogens ; Hydrophobic and Hydrophilic Interactions ; Materials Testing ; Methacrylates / radiation effects ; Mice ; Photochemistry ; Photoinitiators, Dental / pharmacology ; Resins, Synthetic / radiation effects ; para-Aminobenzoates / pharmacology Substance Nomenclature: 0 (Halogens) ; 0 (Methacrylates) ; 0 (Photoinitiators, Dental) ; 0 (Resins, Synthetic) ; 0 (para-Aminobenzoates) ; 454I75YXY0 (Bisphenol A-Glycidyl Methacrylate) ; 6E1I4IV47V (hydroxyethyl methacrylate) ; 76-22-2 (Camphor) ; 829S8D3Y0X (ethyl 4-dimethylaminobenzoate) ; RAL3591W33 (camphorquinone) Entry Date(s): Date Created: 20130706 Date Completed: 20140114 Latest Revision: 20191210 Update Code: 20240513

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Influence of different photo-activation distances on cytotoxicity of a dental adhesive model resin.