Einzeltreffer — DigiBib

Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant. A sensitive and selective indirect competitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) was developed for detecting TBBPA. The optimal hapten of TBBPA was 2-(2,6-dibromo-4-(2-(3,5-dibromo-4-hydroxyphenly)propan-2-yl)) acetic acid. Several physiochemical factors that influence assay performance, such as optimal coupling concentration of immunogen and antibody, organic solvent, ionic strength, and pH, were studied and optimized. The limit of detection (IC10) was 0.027 ng/mL and the median inhibitory concentration (IC50) was 0.58 ng/mL. The BA-ELISA was highly selective, with low cross-reactivity with TBBPA analogs. Finally, the assay was used to detect TBBPA in electronic waste samples. The results are consistent with those using liquid chromatography, which proves that the proposed immunoassay is accurate and receptive. This BA-ELISA method is suitable for the rapid and sensitive screening of TBBPA in environmental monitoring.

Titel:	Biotin-streptavidin enzyme-linked immunosorbent assay for detecting Tetrabromobisphenol A in electronic waste.
Autor/in / Beteiligte Person:	Bu, D ; Zhuang, H ; Zhou, X ; Yang, G
Zeitschrift:	Talanta, Jg. 120 (2014-03-01), S. 40-6
Veröffentlichung:	Amsterdam : Elsevier ; <i>Original Publication</i>: Oxford : Pergamon Press, 2014
Medientyp:	academicJournal
ISSN:	1873-3573 (electronic)
DOI:	10.1016/j.talanta.2013.11.080
Schlagwort:	Biotin chemistry Environmental Monitoring methods Enzyme-Linked Immunosorbent Assay methods Limit of Detection Streptavidin chemistry Electronic Waste analysis Flame Retardants analysis Polybrominated Biphenyls analysis
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't Language: English [Talanta] 2014 Mar; Vol. 120, pp. 40-6. <i>Date of Electronic Publication: </i>2013 Dec 04. MeSH Terms: Electronic Waste / analysis ; Flame Retardants / analysis ; Polybrominated Biphenyls / *analysis ; Biotin / chemistry ; Environmental Monitoring / methods ; Enzyme-Linked Immunosorbent Assay / methods ; Limit of Detection ; Streptavidin / chemistry Contributed Indexing: Keywords: Biotin–streptavidin-amplified system; Electronic waste; Enzyme-linked immunosorbent assay; Tetrabromobisphenol A Substance Nomenclature: 0 (Flame Retardants) ; 0 (Polybrominated Biphenyls) ; 6SO6U10H04 (Biotin) ; 9013-20-1 (Streptavidin) ; FQI02RFC3A (tetrabromobisphenol A) Entry Date(s): Date Created: 20140129 Date Completed: 20140919 Latest Revision: 20191210 Update Code: 20231215

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Biotin-streptavidin enzyme-linked immunosorbent assay for detecting Tetrabromobisphenol A in electronic waste.