Use of an Epstein-Barr virus episomal replicon for anti-sense RNA-mediated gene inhibition in a human cytotoxic T-cell clone.
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 85 (1988-06-01), Heft 11, S. 4010-4
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Zugriff:
A methodology was developed for stable gene transfer into cloned nontransformed human T lymphocytes. Stable high-level gene expression was achieved in cloned human T cells by using a self-replicating Epstein-Barr virus (EBV) episomal replicon. A comparison of five eukaryotic promoters established that the Rous sarcoma virus 3' long terminal repeat (RSV 3' LTR) and the lymphopapilloma virus (LPV) 5' LTR are optimal for episome-based expression in T cells. Effective (greater than 95%), selective, and reversible anti-sense RNA-mediated gene inhibition of a model T-cell-associated molecule (CD8) was achieved in a cytotoxic human T-cell clone by using an EBV episome-based, RSV 3' LTR-driven expression system. The linking of anti-sense RNA mutagenesis and T-cell cloning technologies should contribute significantly to studies of human T-cell function.
| Titel: |
Use of an Epstein-Barr virus episomal replicon for anti-sense RNA-mediated gene inhibition in a human cytotoxic T-cell clone.
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| Autor/in / Beteiligte Person: | Hambor, JE ; Hauer, CA ; Shu, HK ; Groger, RK ; Kaplan, DR ; Tykocinski, ML |
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| Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 85 (1988-06-01), Heft 11, S. 4010-4 |
| Veröffentlichung: | Washington, DC : National Academy of Sciences, 1988 |
| Medientyp: | academicJournal |
| ISSN: | 0027-8424 (print) |
| DOI: | 10.1073/pnas.85.11.4010 |
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