The adaptor protein p62 is involved in RANKL-induced autophagy and osteoclastogenesis.
In: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Jg. 62 (2014-12-01), Heft 12, S. 879-88
Online
academicJournal
Zugriff:
Previous studies have implicated autophagy in osteoclast differentiation. The aim of this study was to investigate the potential role of p62, a characterized adaptor protein for autophagy, in RANKL-induced osteoclastogenesis. Real-time quantitative PCR and western blot analyses were used to evaluate the expression levels of autophagy-related markers during RANKL-induced osteoclastogenesis in mouse macrophage-like RAW264.7 cells. Meanwhile, the potential relationship between p62/LC3 localization and F-actin ring formation was tested using double-labeling immunofluorescence. Then, the expression of p62 in RAW264.7 cells was knocked down using small-interfering RNA (siRNA), followed by detecting its influence on RANKL-induced autophagy activation, osteoclast differentiation, and F-actin ring formation. The data showed that several key autophagy-related markers including p62 were significantly altered during RANKL-induced osteoclast differentiation. In addition, the expression and localization of p62 showed negative correlation with LC3 accumulation and F-actin ring formation, as demonstrated by western blot and immunofluorescence analyses, respectively. Importantly, the knockdown of p62 obviously attenuated RANKL-induced expression of autophagy- and osteoclastogenesis-related genes, formation of TRAP-positive multinuclear cells, accumulation of LC3, as well as formation of F-actin ring. Our study indicates that p62 may play essential roles in RANKL-induced autophagy and osteoclastogenesis, which may help to develop a novel therapeutic strategy against osteoclastogenesis-related diseases.
(© The Author(s) 2014.)
Titel: |
The adaptor protein p62 is involved in RANKL-induced autophagy and osteoclastogenesis.
|
---|---|
Autor/in / Beteiligte Person: | Li, RF ; Chen, G ; Ren, JG ; Zhang, W ; Wu, ZX ; Liu, B ; Zhao, Y ; Zhao, YF |
Link: | |
Zeitschrift: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Jg. 62 (2014-12-01), Heft 12, S. 879-88 |
Veröffentlichung: | <2011->: Thousand Oaks, CA : SAGE Publications ; <i>Original Publication</i>: Baltimore : Williams & Wilkins Co., c1953-, 2014 |
Medientyp: | academicJournal |
ISSN: | 1551-5044 (electronic) |
DOI: | 10.1369/0022155414551367 |
Schlagwort: |
|
Sonstiges: |
|