Characterization of zeta (zeta): a new opioid receptor involved in growth.
In: Brain research, Jg. 482 (1989-03-20), Heft 2, S. 297-305
academicJournal
Zugriff:
Endogenous opioid systems (i.e., opioids and opioid receptors) are known to play a role in neural cancer. Using [3H]-[Met5]enkephalin, a potent ligand involved in growth, specific and saturable binding was detected in homogenates of S20Y neuroblastoma transplanted into A/Jax mice; the data fit a single binding site. Scatchard analysis yielded a Kd of 0.49 nM and a binding capacity of 5.32 fmol/mg protein. Binding was dependent on protein concentration, time, temperature, and pH, and was sensitive to Na+ and guanine nucleotides. Optimal binding required protease inhibitors, and pretreatment of the tumor homogenates with trypsin markedly reduced [3H]-[Met5]enkephalin binding, suggesting that the binding site was proteinaceous in character. Displacement experiments indicated that [Met5]enkephalin was the most potent displacer of [3H]-[Met5]enkephalin; other ligands selective for mu, delta, kappa, epsilon, and sigma were not highly competitive. Given the functional significance of [Met5]enkephalin as a potent regulator of normal and abnormal growth, and that the receptor recognized by [Met5]enkephalin does not resemble any previously described, the present study has demonstrated the presence of a new opioid receptor termed zeta (zeta) (from the Greek 'Zoe', life) related to the proliferation of cells and tissues.
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Characterization of zeta (zeta): a new opioid receptor involved in growth.
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Autor/in / Beteiligte Person: | Zagon, IS ; Goodman, SR ; McLaughlin, PJ |
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Zeitschrift: | Brain research, Jg. 482 (1989-03-20), Heft 2, S. 297-305 |
Veröffentlichung: | Amsterdam Elsevier/North-Holland Biomedical Press., 1989 |
Medientyp: | academicJournal |
ISSN: | 0006-8993 (print) |
DOI: | 10.1016/0006-8993(89)91192-x |
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