Polymethoxylated flavones potentiate the cytolytic activity of NK leukemia cell line KHYG-1 via enhanced expression of granzyme B.

Saito, T ; Abe, D ; et al.

In: Biochemical and biophysical research communications, Jg. 456 (2015-01-16), Heft 3, S. 799-803

academicJournal

Polymethoxylated flavones (PMFs) are found in the peel tissues of some citrus species. Here, we report that PMFs, such as nobiletin, potentiate the cytolytic activity of KHYG-1 natural killer (NK) leukemia cells. Nobiletin markedly enhanced the expression of granzyme B, a serine protease that plays critical roles in the cytolytic activity of NK cells. The potentiated cytolytic activity induced by nobiletin was canceled by the granzyme B inhibitor Z-AAD-CMK. Nobiletin also increased the levels of phosphorylated CREB, ERK1/2, and p38 MAPK in KHYG-1 cells, which are known to participate in NK cell function. Inhibition of an upstream kinase of ERK1/2 failed to reduce the granzyme B expression and KHYG-1 cytolytic activity. Meanwhile, inhibition of p38 MAPK attenuated both granzyme B expression and KHYG-1 cytolytic activity. These results suggest that the primary role of nobiletin in KHYG-1 cytolytic activity lies in upregulation of granzyme B expression, at least in part, mediated through p38 MAPK function.

Titel:	Polymethoxylated flavones potentiate the cytolytic activity of NK leukemia cell line KHYG-1 via enhanced expression of granzyme B.
Autor/in / Beteiligte Person:	Saito, T ; Abe, D ; Nogata, Y
Zeitschrift:	Biochemical and biophysical research communications, Jg. 456 (2015-01-16), Heft 3, S. 799-803
Veröffentlichung:	<2002- >: San Diego, CA : Elsevier ; <i>Original Publication</i>: New York, Academic Press., 2015
Medientyp:	academicJournal
ISSN:	1090-2104 (electronic)
DOI:	10.1016/j.bbrc.2014.12.027
Schlagwort:	Amino Acid Chloromethyl Ketones pharmacology Cell Line, Tumor Citrus chemistry Flavones isolation & purification Granzymes antagonists & inhibitors Humans Killer Cells, Natural enzymology p38 Mitogen-Activated Protein Kinases metabolism Flavones pharmacology Granzymes metabolism Killer Cells, Natural drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biochem Biophys Res Commun] 2015 Jan 16; Vol. 456 (3), pp. 799-803. <i>Date of Electronic Publication: </i>2014 Dec 13. MeSH Terms: Flavones / pharmacology ; Granzymes / metabolism ; Killer Cells, Natural / *drug effects ; Amino Acid Chloromethyl Ketones / pharmacology ; Cell Line, Tumor ; Citrus / chemistry ; Flavones / isolation & purification ; Granzymes / antagonists & inhibitors ; Humans ; Killer Cells, Natural / enzymology ; p38 Mitogen-Activated Protein Kinases / metabolism Contributed Indexing: Keywords: Granzyme B; KHYG-1; NK cell; Nobiletin; Polymethoxylated flavone; p38 MAPK Substance Nomenclature: 0 (Amino Acid Chloromethyl Ketones) ; 0 (Flavones) ; 0 (Z-Ala-Ala-Asp-chloromethylketone) ; D65ILJ7WLY (nobiletin) ; EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) ; EC 3.4.21.- (Granzymes) Entry Date(s): Date Created: 20141217 Date Completed: 20150320 Latest Revision: 20151119 Update Code: 20231215

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.