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Chloroquine resistance (CQR) in falciparum malaria was identified to be associated with several mutations in the chloroquine resistance transporter gene (pfcrt) that encodes the transmembrane transporter in digestive vacuole membrane of the parasite. This study aimed to investigate the point mutations across the full-length pfcrt in Plasmodium falciparum isolates in Sabah, Malaysia. A total of 31 P. falciparum positive samples collected from Keningau, Kota Kinabalu, and Kudat, Sabah, were analyzed. pfcrt was PCR amplified and cloned prior to sequence analysis. This study showed that all the previously described 10 point mutations associated with CQR at codons 72, 74, 75, 76, 97, 220, 271, 326, 356, and 371 were found with different prevalence. Besides, two novel point mutations, I166V and H273N, were identified with 22.5% and 19.3%, respectively. Three haplotypes, namely, CVMNK (29%), CVIET (3.2%), and SVMNT (67.7%), were identified. High prevalence of SVMNT among P. falciparum isolates from Sabah showed that these isolates are closer to the P. falciparum isolates from Papua New Guinea rather than to the more proximal Southeast Asian CVIET haplotype. Full-length analysis of pfcrt showed that chloroquine resistant P. falciparum in Sabah is still prevalent despite the withdrawal of chloroquine usage since 1979.

Titel:	Full-length sequence analysis of chloroquine resistance transporter gene in Plasmodium falciparum isolates from Sabah, Malaysia.
Autor/in / Beteiligte Person:	Tan, LL ; Lau, TY ; Timothy, W ; Prabakaran, D
Link:	Full Text Finder (Volltext)
Zeitschrift:	TheScientificWorldJournal, Jg. 2014 (2014), S. 935846
Veröffentlichung:	New York : Hindawi Publishing Corporation ; <i>Original Publication</i>: Boynton Beach, FL : Scientific World, Inc., c2001-, 2014
Medientyp:	academicJournal
ISSN:	1537-744X (electronic)
DOI:	10.1155/2014/935846
Schlagwort:	Electrophoresis, Agar Gel Geography Haplotypes genetics Malaysia Point Mutation genetics Polymerase Chain Reaction Drug Resistance genetics Membrane Transport Proteins genetics Plasmodium falciparum genetics Plasmodium falciparum isolation & purification Protozoan Proteins genetics Sequence Analysis, DNA
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [ScientificWorldJournal] 2014; Vol. 2014, pp. 935846. <i>Date of Electronic Publication: </i>2014 Oct 28. MeSH Terms: Sequence Analysis, DNA* ; Drug Resistance / genetics ; Membrane Transport Proteins / genetics ; Plasmodium falciparum / genetics ; Plasmodium falciparum / isolation & purification ; Protozoan Proteins / *genetics ; Electrophoresis, Agar Gel ; Geography ; Haplotypes / genetics ; Malaysia ; Point Mutation / genetics ; Polymerase Chain Reaction References: Mol Cell. 2000 Oct;6(4):861-71. (PMID: 11090624) ; Malar J. 2012;11:251. (PMID: 22853645) ; J Infect Dis. 2001 May 15;183(10):1543-5. (PMID: 11319698) ; Ann Trop Med Parasitol. 2001 Sep;95(6):559-72. (PMID: 11672462) ; Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12689-94. (PMID: 11675500) ; Nature. 2002 Jul 18;418(6895):320-3. (PMID: 12124623) ; Science. 2002 Oct 4;298(5591):210-3. (PMID: 12364805) ; Int J Parasitol. 2003 Nov;33(13):1545-52. (PMID: 14572517) ; Antimicrob Agents Chemother. 2003 Nov;47(11):3500-5. (PMID: 14576108) ; Am J Trop Med Hyg. 2004 Jul;71(1):40-2. (PMID: 15238686) ; Mol Biochem Parasitol. 2004 Aug;136(2):273-85. (PMID: 15478806) ; Med J Malaya. 1966 Dec;21(2):115-24. (PMID: 4227380) ; Trans R Soc Trop Med Hyg. 1976;70(2):145-8. (PMID: 785725) ; J Trop Med Hyg. 1980 Dec;83(6):259-64. (PMID: 7003166) ; Mol Biochem Parasitol. 1993 Oct;61(2):315-20. (PMID: 8264734) ; Trans R Soc Trop Med Hyg. 1996 May-Jun;90(3):294-7. (PMID: 8758083) ; Trans R Soc Trop Med Hyg. 1963 Nov;57:409-16. (PMID: 14081295) ; Antimicrob Agents Chemother. 2005 May;49(5):2102-5. (PMID: 15855538) ; Acta Trop. 2005 Jun;94(3):170-80. (PMID: 15866507) ; Malar J. 2008;7:61. (PMID: 18423045) ; Acta Trop. 2009 Jan;109(1):37-40. (PMID: 18950599) ; Parasitol Int. 2009 Jun;58(2):166-70. (PMID: 19567229) ; Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):18883-9. (PMID: 19884511) ; Malar J. 2011;10:42. (PMID: 21320353) ; Southeast Asian J Trop Med Public Health. 2011 Nov;42(6):1322-6. (PMID: 22299399) ; Mem Inst Oswaldo Cruz. 2012 Feb;107(1):129-34. (PMID: 22310546) ; N Engl J Med. 2001 Jan 25;344(4):257-63. (PMID: 11172152) Substance Nomenclature: 0 (Membrane Transport Proteins) ; 0 (PfCRT protein, Plasmodium falciparum) ; 0 (Protozoan Proteins) Entry Date(s): Date Created: 20150110 Date Completed: 20151109 Latest Revision: 20181113 Update Code: 20240513 PubMed Central ID: PMC4276362

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Full-length sequence analysis of chloroquine resistance transporter gene in Plasmodium falciparum isolates from Sabah, Malaysia.