Mapping antigenic motifs in the trypomastigote small surface antigen from Trypanosoma cruzi.
In: Clinical and vaccine immunology : CVI, Jg. 22 (2015-03-01), Heft 3, S. 304-12
Online
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Zugriff:
The trypomastigote small surface antigen (TSSA) is a mucin-like molecule from Trypanosoma cruzi, the etiological agent of Chagas disease, which displays amino acid polymorphisms in parasite isolates. TSSA expression is restricted to the surface of infective cell-derived trypomastigotes, where it functions as an adhesin and engages surface receptors on the host cell as a prerequisite for parasite internalization. Previous results have established TSSA-CL, the isoform encoded by the CL Brener clone, as an appealing candidate for use in serology-based diagnostics for Chagas disease. Here, we used a combination of peptide- and recombinant protein-based tools to map the antigenic structure of TSSA-CL at maximal resolution. Our results indicate the presence of different partially overlapping B-cell epitopes clustering in the central portion of TSSA-CL, which contains most of the polymorphisms found in parasite isolates. Based on these results, we assessed the serodiagnostic performance of a 21-amino-acid-long peptide that spans TSSA-CL major antigenic determinants, which was similar to the performance of the previously validated glutathione S-transferase (GST)-TSSA-CL fusion molecule. Furthermore, the tools developed for the antigenic characterization of the TSSA antigen were also used to explore other potential diagnostic applications of the anti-TSSA humoral response in Chagasic patients. Overall, our present results provide additional insights into the antigenic structure of TSSA-CL and support this molecule as an excellent target for molecular intervention in Chagas disease.
(Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
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Mapping antigenic motifs in the trypomastigote small surface antigen from Trypanosoma cruzi.
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Autor/in / Beteiligte Person: | Balouz, V ; Cámara Mde, L ; Cánepa, GE ; Carmona, SJ ; Volcovich, R ; Gonzalez, N ; Altcheh, J ; Agüero, F ; Buscaglia, CA |
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Zeitschrift: | Clinical and vaccine immunology : CVI, Jg. 22 (2015-03-01), Heft 3, S. 304-12 |
Veröffentlichung: | Washington, D.C. : American Society for Microbiology, [2006]-, 2015 |
Medientyp: | academicJournal |
ISSN: | 1556-679X (electronic) |
DOI: | 10.1128/CVI.00684-14 |
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