[Effect of Guishen Pill on expression levels of Oct-4, MVH, and Egr-1 in mice with diminished ovarian reserve].

Cui, DD ; Ma, WW ; et al.

In: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine, Jg. 35 (2015), Heft 1, S. 76-80

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Objective: To study the effect of Guishen Pill (GSP) on expression levels of Oct-4, MVH, and Egr-1 in mice with diminished ovarian reserve (DOR).

Methods: Totally 40 female C57BL/6J mice were randomly divided into 4 groups, the normal control group, the model group, the GSP group, and the dehydroepiandrosterone (DHEA) group, 10 in each group. Pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG), and prostaglandin F2α (PGF2α) were sequentially administrated to produce superovulation. The DOR model was established by exposing to ozone inhalation. Mice in the GSP group were intragastrically administered with GSP at 0.3 mL. Those in the DHEA group were intragastrically administered with DHEA at 0.3 mL. Equal volume of normal saline was intragastrically administered to mice in the normal control group and the model group. All mice wer treated for 21 days. Serum levels of estrogen (E2), progestogen (P), and anti-Müllerian hormone (AMH) were measured by ELISA. Changes of Oct-4, anti-AMH, and early growth response gene-1 (Egr-1) mRNA in ovaries were dtected by Real-time PCR.

Results: Compared with the model group, serum levels of E2, P, and AMH, as well as contents of estrogen receptor (ER), progestogen receptor (PR), MVH, and Oct-4 mRNA significantly increased in the GSP group and the DHEA group (P < 0.05).

Conclusion: GSP could improve expression levels of Oct-4, MVH, and Egr-1 mRNA in DOR mice and their ovarian function.

Titel:	[Effect of Guishen Pill on expression levels of Oct-4, MVH, and Egr-1 in mice with diminished ovarian reserve].
Autor/in / Beteiligte Person:	Cui, DD ; Ma, WW ; Wen, L ; Song, KK ; Ding, JH ; Huang, C ; Zhang, MM
Zeitschrift:	Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine, Jg. 35 (2015), Heft 1, S. 76-80
Veröffentlichung:	Beijing : Gai yan jiu yuan ; <i>Original Publication</i>: Beijing : Gai yan jiu yuan, 1992-, 2015
Medientyp:	academicJournal
ISSN:	1003-5370 (print)
Schlagwort:	Animals Anti-Mullerian Hormone metabolism Dehydroepiandrosterone metabolism Estrogens Female Mice Mice, Inbred C57BL Ovarian Reserve Ovary Pregnancy Superovulation Drugs, Chinese Herbal pharmacology Early Growth Response Protein 1 metabolism Octamer Transcription Factor-3 metabolism Receptors, Estrogen metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: Chinese Publication Type: Journal Article Language: Chinese [Zhongguo Zhong Xi Yi Jie He Za Zhi] 2015 Jan; Vol. 35 (1), pp. 76-80. MeSH Terms: Drugs, Chinese Herbal / pharmacology ; Early Growth Response Protein 1 / metabolism ; Octamer Transcription Factor-3 / metabolism ; Receptors, Estrogen / metabolism ; Animals ; Anti-Mullerian Hormone / metabolism ; Dehydroepiandrosterone / metabolism ; Estrogens ; Female ; Mice ; Mice, Inbred C57BL ; Ovarian Reserve ; Ovary ; Pregnancy ; Superovulation Substance Nomenclature: 0 (Drugs, Chinese Herbal) ; 0 (Early Growth Response Protein 1) ; 0 (Egr1 protein, mouse) ; 0 (Estrogens) ; 0 (Octamer Transcription Factor-3) ; 0 (Receptors, Estrogen) ; 459AG36T1B (Dehydroepiandrosterone) ; 80497-65-0 (Anti-Mullerian Hormone) Entry Date(s): Date Created: 20150321 Date Completed: 20150908 Latest Revision: 20181202 Update Code: 20240513

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