Promotion of classic neutral bile acids synthesis pathway is responsible for cholesterol-lowing effect of Si-miao-yong-an decoction: Application of LC-MS/MS method to determine 6 major bile acids in rat liver and plasma.

Liu, Z ; Zhang, Y ; et al.

In: Journal of pharmaceutical and biomedical analysis, Jg. 135 (2017-02-20), S. 167-175

academicJournal

Si-miao-yong-an decoction (SMYAD), a traditional Chinese medicine formula, significantly reduced plasma TC, LDL-c levels and increased HDL-c level in hyperlipidemia rats. Liver function test and tissue section examination indicated that SMYAD improved liver function and reduced fat accumulation in hyperlipidemia rat liver. A LC-MS/MS method was established and well validated to evaluate major bile acids derived from cholesterol metabolism through the classic neutral pathway and the alternative acidic pathway (cholic acid, chenodeoxycholic acid and their taurine and glycine conjugates) in liver and plasma. Increased total 6 bile acids concentrations in both liver and plasma were observed after oral administration of 12g/kg/d, 24g/kg/d and 36g/kg/d of SMYAD in a dose dependent manner which contributed to eliminate of cholesterol. Cholic acid, taurocholic acid and glycocholic acid act as the main products of bile acid classic neutral synthesis pathway and show sharp increase (p<0.01) after treatment of SMYAD at dosage of 24-36g/kg/d. For liver samples, taurocholic acid level act as the largest growth section, while in plasma samples, cholic acid act as the largest growth section after SMYAD treatment, compared with Model group. By contrast, the main products of alternative acidic pathway (chenodeoxycholic acid and its glycine and taurine conjugates) show no significant increase after treatment of SMYAD. In conclusion, the cholesterol lowing effect of SMYAD may be related with the accelerated transformation of cholesterol into bile acids through the classic neutral pathway.

Titel:	Promotion of classic neutral bile acids synthesis pathway is responsible for cholesterol-lowing effect of Si-miao-yong-an decoction: Application of LC-MS/MS method to determine 6 major bile acids in rat liver and plasma.
Autor/in / Beteiligte Person:	Liu, Z ; Zhang, Y ; Zhang, R ; Gu, L ; Chen, X
Zeitschrift:	Journal of pharmaceutical and biomedical analysis, Jg. 135 (2017-02-20), S. 167-175
Veröffentlichung:	<2006->: London : Elsevier Science ; <i>Original Publication</i>: Oxford ; New York : Pergamon Press, c1983-, 2017
Medientyp:	academicJournal
ISSN:	1873-264X (electronic)
DOI:	10.1016/j.jpba.2016.12.021
Schlagwort:	Administration, Oral Animals Anticholesteremic Agents administration & dosage Anticholesteremic Agents blood Bile Acids and Salts analysis Bile Acids and Salts blood Cholesterol blood Cholesterol metabolism Drugs, Chinese Herbal administration & dosage Liver drug effects Male Metabolic Networks and Pathways drug effects Metabolic Networks and Pathways physiology Plasma drug effects Plasma metabolism Random Allocation Rats Rats, Sprague-Dawley Anticholesteremic Agents metabolism Bile Acids and Salts metabolism Chromatography, Liquid methods Drugs, Chinese Herbal metabolism Liver metabolism Tandem Mass Spectrometry methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Pharm Biomed Anal] 2017 Feb 20; Vol. 135, pp. 167-175. <i>Date of Electronic Publication: </i>2016 Dec 18. MeSH Terms: Anticholesteremic Agents / metabolism ; Bile Acids and Salts / metabolism ; Chromatography, Liquid / methods ; Drugs, Chinese Herbal / metabolism ; Liver / metabolism ; Tandem Mass Spectrometry / methods ; Administration, Oral ; Animals ; Anticholesteremic Agents / administration & dosage ; Anticholesteremic Agents / blood ; Bile Acids and Salts / analysis ; Bile Acids and Salts / blood ; Cholesterol / blood ; Cholesterol / metabolism ; Drugs, Chinese Herbal / administration & dosage ; Liver / drug effects ; Male ; Metabolic Networks and Pathways / drug effects ; Metabolic Networks and Pathways / physiology ; Plasma / drug effects ; Plasma / metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley Contributed Indexing: Keywords: Bile acids; Hyperlipidemia; LC–MS/MS; Si-miao-yong-an decoction Substance Nomenclature: 0 (Anticholesteremic Agents) ; 0 (Bile Acids and Salts) ; 0 (Drugs, Chinese Herbal) ; 97C5T2UQ7J (Cholesterol) Entry Date(s): Date Created: 20161230 Date Completed: 20170524 Latest Revision: 20171208 Update Code: 20231215

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