Montmorillonite-chitosan-chlorhexidine composite films with antibiofilm activity and improved cytotoxicity for wound dressing.
In: Journal of colloid and interface science, Jg. 491 (2017-04-01), S. 265-272
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Zugriff:
Hypothesis: Chlorhexidine (CLX) is a good antimicrobial agent, but its use in treatment of wounds is limited because of its cytotoxicity towards human fibroblasts. A delivery system, able to release CLX in a localized and prolonged manner, could guarantee antimicrobial activity with reduced cytotoxic. Thus in this work the preparation and characterization of chitosan/montmorillonite composite films containing CLX, able to offer a prolonged CLX release, is described. The antimicrobial and antibiofilm activities and cytotoxicity of films were investigated.
Experimental: CLX was intercalated between the layers of montmorillonite (MONT-Na), and the intercalated product (MONT-CLX) was characterized by X-ray powder diffraction (XRPD), thermogravimetric analysis (TGA) and FT-IR spectroscopy. Then chitosan/MONT-CLX films were prepared and characterized. For comparison, films loaded with neat CLX and MONT-Na/CLX were prepared. All prepared films were tested for their antimicrobial and antibiofilm activities. Cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe was evaluated as well.
Findings: All prepared films showed good antimicrobial and antibiofilm activities. As concerns cytotoxicity the film containing MONT-CLX at 1% CLX concentration resulted no cytotoxic. These results confirm the potential use of chitosan films containing MONT-CLX as a potential wound dressing material to prevent microbial colonization in wounds.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Titel: |
Montmorillonite-chitosan-chlorhexidine composite films with antibiofilm activity and improved cytotoxicity for wound dressing.
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Autor/in / Beteiligte Person: | Ambrogi, V ; Pietrella, D ; Nocchetti, M ; Casagrande, S ; Moretti, V ; De Marco, S ; Ricci, M |
Zeitschrift: | Journal of colloid and interface science, Jg. 491 (2017-04-01), S. 265-272 |
Veröffentlichung: | Orlando, FL : Academic Press ; <i>Original Publication</i>: New York., 2017 |
Medientyp: | academicJournal |
ISSN: | 1095-7103 (electronic) |
DOI: | 10.1016/j.jcis.2016.12.058 |
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