Einzeltreffer — DigiBib

Fremyella diplosiphon is a freshwater cyanobacterium that has great potential as a biofuel agent due to its ability to grow in low light intensity and acclimation to different wavelengths. To enhance its halotolerance for growth in 35gL -1 sodium chloride (NaCl), plasmids harboring hemolysin B (hlyB) and malate dehydrogenase (mdh) genes were transformed into wild type F. diplosiphon (WT-Fd33). Electroporation-mediated overexpression of the genes resulted in two transformants, HSF33-1 and HSF33-2, with 9- and 20-fold increases in hlyB and mdh transcript levels. In addition, up-regulation of proteins at the expected size ranges of 50-60kDa for HlyB and 40-50kDa for MDH was observed. Two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry revealed a protein spot corresponding to HlyB in HSF33-1 with a significant MOWSE score of 164 and 3% sequence coverage, and a spot corresponding to MDH in HSF33-2 gave a significant MOWSE score of 124 with 10% sequence coverage. Physiological evaluation in BG11/HEPES medium and seawater adjusted to 35gL -1 NaCl confirmed that the transformants could thrive in high salinity with no loss of photosynthetic pigments. Results of the study indicate that overexpression of hlyB and mdh genes confer halotolerance in F. diplosiphon, thus maximizing its potential as a large-scale biofuel agent.

Titel:	Overexpression of hlyB and mdh genes confers halotolerance in Fremyella diplosiphon, a freshwater cyanobacterium.
Autor/in / Beteiligte Person:	Tabatabai, B ; Arumanayagam, AS ; Enitan, O ; Mani, A ; Natarajan, SS ; Sitther, V
Zeitschrift:	Enzyme and microbial technology, Jg. 103 (2017-08-01), S. 12-17
Veröffentlichung:	New York, NY : Elsevier ; <i>Original Publication</i>: [Guildford, Eng.] IPC Science and Technology Press., 2017
Medientyp:	academicJournal
ISSN:	1879-0909 (electronic)
DOI:	10.1016/j.enzmictec.2017.04.009
Schlagwort:	ATP-Binding Cassette Transporters metabolism Bacterial Proteins genetics Bacterial Proteins metabolism Biofuels Cyanobacteria growth & development Cyanobacteria metabolism Electrophoresis, Gel, Two-Dimensional Fresh Water microbiology Genes, Bacterial Hemolysin Proteins metabolism Industrial Microbiology Malate Dehydrogenase metabolism Salinity Up-Regulation ATP-Binding Cassette Transporters genetics Cyanobacteria genetics Hemolysin Proteins genetics Malate Dehydrogenase genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Enzyme Microb Technol] 2017 Aug; Vol. 103, pp. 12-17. <i>Date of Electronic Publication: </i>2017 Apr 24. MeSH Terms: ATP-Binding Cassette Transporters / genetics ; Cyanobacteria / genetics ; Hemolysin Proteins / genetics ; Malate Dehydrogenase / genetics ; ATP-Binding Cassette Transporters / metabolism ; Bacterial Proteins / genetics ; Bacterial Proteins / metabolism ; Biofuels ; Cyanobacteria / growth & development ; Cyanobacteria / metabolism ; Electrophoresis, Gel, Two-Dimensional ; Fresh Water / microbiology ; Genes, Bacterial ; Hemolysin Proteins / metabolism ; Industrial Microbiology ; Malate Dehydrogenase / metabolism ; Salinity ; Up-Regulation Contributed Indexing: Keywords: Genetic transformation; MALDI/TOF/TOF MS; Reverse transcription-quantitative PCR; Salinity stress Substance Nomenclature: 0 (ATP-Binding Cassette Transporters) ; 0 (Bacterial Proteins) ; 0 (Biofuels) ; 0 (Hemolysin Proteins) ; EC 1.1.1.37 (Malate Dehydrogenase) ; EC 3.6.1.3 (hemolysin B) Entry Date(s): Date Created: 20170531 Date Completed: 20180402 Latest Revision: 20180402 Update Code: 20240513

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Overexpression of hlyB and mdh genes confers halotolerance in Fremyella diplosiphon, a freshwater cyanobacterium.