Ectopic delivery of miR-200c diminishes hepatitis C virus infectivity through transcriptional and translational repression of Occludin.

Elhelw, DS ; Riad, SE ; et al.

In: Archives of virology, Jg. 162 (2017-11-01), Heft 11, S. 3283-3291

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Zugriff:

Occludin (OCLN) is an essential factor for HCV entry through interacting with other surface receptors. The aim of this study was to investigate the epigenetic regulation of Occludin expression and to study its impact on viral infectivity. microRNAs expression was assessed using qRT-PCR, while OCLN protein expression was investigated by indirect immunofluorescence and Western blotting. Viral infectivity was assessed by measuring viral-load using qRT-PCR. In silico analysis predicted that miR-200c targeted the OCLN 3'UTR, which was further experimentally confirmed. miR-122 was previously validated to target the 3'UTR of OCLN and was used as a control. We report a significant down-regulation of miR-200c in liver tissues of HCV-infected patients. Ectopic expression of both miR-122 and miR-200c in Huh7 cells reduced OCLN mRNA and protein levels. Viral infectivity was significantly reduced by miR-200c but enhanced by miR-122. This work sheds light on miR-200c as a novel regulator of HCV infectivity through the regulation of OCLN.

Titel:	Ectopic delivery of miR-200c diminishes hepatitis C virus infectivity through transcriptional and translational repression of Occludin.
Autor/in / Beteiligte Person:	Elhelw, DS ; Riad, SE ; Shawer, H ; El-Ekiaby, N ; Salah, A ; Zekri, A ; Amleh, A ; Esmat, G ; Abdelaziz, AI
Link:	View record at Springer (Volltext)
Zeitschrift:	Archives of virology, Jg. 162 (2017-11-01), Heft 11, S. 3283-3291
Veröffentlichung:	Wien, New York, Springer-Verlag., 2017
Medientyp:	academicJournal
ISSN:	1432-8798 (electronic)
DOI:	10.1007/s00705-017-3449-3
Schlagwort:	Cell Line, Tumor Hepatocytes metabolism Hepatocytes virology Humans MicroRNAs genetics MicroRNAs metabolism Occludin genetics RNA, Viral Virus Replication Gene Expression Regulation drug effects Hepacivirus physiology Hepatocytes drug effects MicroRNAs pharmacology Occludin metabolism Protein Biosynthesis drug effects Transcription, Genetic drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Arch Virol] 2017 Nov; Vol. 162 (11), pp. 3283-3291. <i>Date of Electronic Publication: </i>2017 Jun 22. MeSH Terms: Gene Expression Regulation / drug effects ; Hepacivirus / physiology ; Hepatocytes / drug effects ; MicroRNAs / pharmacology ; Occludin / metabolism ; Protein Biosynthesis / drug effects ; Transcription, Genetic / *drug effects ; Cell Line, Tumor ; Hepatocytes / metabolism ; Hepatocytes / virology ; Humans ; MicroRNAs / genetics ; MicroRNAs / metabolism ; Occludin / genetics ; RNA, Viral ; Virus Replication Comments: Erratum in: Arch Virol. 2018 Mar 24;:. (PMID: 29572597) Substance Nomenclature: 0 (MIRN122 microRNA, human) ; 0 (MIRN200 microRNA, human) ; 0 (MicroRNAs) ; 0 (Occludin) ; 0 (RNA, Viral) Entry Date(s): Date Created: 20170624 Date Completed: 20171025 Latest Revision: 20180324 Update Code: 20231215

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