Einzeltreffer — DigiBib

Retroviruses and Long Terminal Repeat (LTR)-retrotransposons have distinct patterns of integration sites. The oncogenic potential of retrovirus-based vectors used in gene therapy is dependent on the selection of integration sites associated with promoters. The LTR-retrotransposon Tf1 of Schizosaccharomyces pombe is studied as a model for oncogenic retroviruses because it integrates into the promoters of stress response genes. Although integrases (INs) encoded by retroviruses and LTR-retrotransposons are responsible for catalyzing the insertion of cDNA into the host genome, it is thought that distinct host factors are required for the efficiency and specificity of integration. We tested this hypothesis with a genome-wide screen of host factors that promote Tf1 integration. By combining an assay for transposition with a genetic assay that measures cDNA recombination we could identify factors that contribute differentially to integration. We utilized this assay to test a collection of 3,004 S. pombe strains with single gene deletions. Using these screens and immunoblot measures of Tf1 proteins, we identified a total of 61 genes that promote integration. The candidate integration factors participate in a range of processes including nuclear transport, transcription, mRNA processing, vesicle transport, chromatin structure and DNA repair. Two candidates, Rhp18 and the NineTeen complex were tested in two-hybrid assays and were found to interact with Tf1 IN. Surprisingly, a number of pathways we identified were found previously to promote integration of the LTR-retrotransposons Ty1 and Ty3 in Saccharomyces cerevisiae, indicating the contribution of host factors to integration are common in distantly related organisms. The DNA repair factors are of particular interest because they may identify the pathways that repair the single stranded gaps flanking the sites of strand transfer following integration of LTR retroelements.

Titel:	Host factors that promote retrotransposon integration are similar in distantly related eukaryotes.
Autor/in / Beteiligte Person:	Rai, SK ; Sangesland, M ; Lee M Jr ; Esnault, C ; Cui, Y ; Chatterjee, AG ; Levin, HL
Link:	Volltext (PDF)
Zeitschrift:	PLoS genetics, Jg. 13 (2017-12-12), Heft 12, S. e1006775
Veröffentlichung:	San Francisco, CA : Public Library of Science, c2005-, 2017
Medientyp:	academicJournal
ISSN:	1553-7404 (electronic)
DOI:	10.1371/journal.pgen.1006775
Schlagwort:	DNA Repair genetics Eukaryota genetics Gene Expression Regulation, Fungal Integrases genetics Promoter Regions, Genetic RNA-Directed DNA Polymerase genetics Retroviridae genetics Saccharomyces cerevisiae genetics Saccharomyces cerevisiae Proteins genetics Schizosaccharomyces genetics Integration Host Factors genetics Recombination, Genetic Retroelements genetics Terminal Repeat Sequences genetics Ubiquitin-Protein Ligases genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural Language: English [PLoS Genet] 2017 Dec 12; Vol. 13 (12), pp. e1006775. <i>Date of Electronic Publication: </i>2017 Dec 12 (<i>Print Publication: </i>2017). MeSH Terms: Recombination, Genetic* ; Integration Host Factors / genetics ; Retroelements / genetics ; Terminal Repeat Sequences / genetics ; Ubiquitin-Protein Ligases / genetics ; DNA Repair / genetics ; Eukaryota / genetics ; Gene Expression Regulation, Fungal ; Integrases / genetics ; Promoter Regions, Genetic ; RNA-Directed DNA Polymerase / genetics ; Retroviridae / genetics ; Saccharomyces cerevisiae / genetics ; Saccharomyces cerevisiae Proteins / genetics ; Schizosaccharomyces / genetics References: Nat Struct Mol Biol. 2015 Aug;22(8):611-7. (PMID: 26167880) ; Genes Dev. 2015 Nov 1;29(21):2287-97. (PMID: 26545813) ; AIDS Res Hum Retroviruses. 2012 Oct;28(10):1329-39. (PMID: 22404213) ; Genetics. 2008 Jan;178(1):197-214. (PMID: 18202368) ; Genetics. 2010 Aug;185(4):1193-205. (PMID: 20498295) ; Curr Top Microbiol Immunol. 1992;176:195-211. (PMID: 1376225) ; J Virol. 2003 May;77(9):5451-63. (PMID: 12692246) ; J Biol Chem. 1997 Sep 12;272(37):23360-5. (PMID: 9287349) ; Mol Cell. 2010 Mar 26;37(6):865-78. (PMID: 20347428) ; Nat Cell Biol. 2009 May;11(5):592-603. (PMID: 19396164) ; J Biol Chem. 2010 Apr 30;285(18):13797-806. (PMID: 20200159) ; Mol Cell Biol. 1999 Aug;19(8):5352-62. (PMID: 10409726) ; Genetics. 1998 Apr;148(4):1743-61. (PMID: 9560391) ; Microbiol Spectr. 2015 Apr;3(2):MDNA3-0057-2014. (PMID: 26104707) ; Mol Cell Biol. 1995 Jun;15(6):3310-7. (PMID: 7760826) ; Methods Mol Biol. 2016;1400:117-30. (PMID: 26895050) ; Nature. 2015 May 14;521(7551):227-31. (PMID: 25731161) ; Genetics. 1999 Apr;151(4):1393-407. (PMID: 10101165) ; Eukaryot Cell. 2002 Feb;1(1):44-55. (PMID: 12455970) ; Cell. 1989 Jun 30;57(7):1275-83. (PMID: 2500253) ; Genetics. 2000 Feb;154(2):543-56. (PMID: 10655210) ; Science. 2001 Aug 10;293(5532):1129-33. (PMID: 11498589) ; Clin Microbiol Infect. 2016 Apr;22(4):324-332. (PMID: 27107301) ; Nat Struct Mol Biol. 2010 May;17 (5):620-8. (PMID: 20418882) ; Mol Cell. 2006 Feb 3;21(3):379-91. (PMID: 16455493) ; Nucleic Acids Res. 2000 Dec 1;28(23):4709-16. (PMID: 11095681) ; Eukaryot Cell. 2006 Oct;5(10):1760-9. (PMID: 17031000) ; Microbiol Spectr. 2014 Oct;2(5):null. (PMID: 26104374) ; Mol Biol Cell. 2007 Sep;18(9):3692-708. (PMID: 17615301) ; Microbiol Mol Biol Rev. 2002 Dec;66(4):630-70, table of contents. (PMID: 12456786) ; Microbiol Spectr. 2015 Apr 1;3(2):1-35. (PMID: 25893143) ; PLoS Pathog. 2013;9(10):e1003693. (PMID: 24130490) ; Cell Host Microbe. 2010 Mar 18;7(3):221-33. (PMID: 20227665) ; Methods Enzymol. 1997;283:141-56. (PMID: 9251017) ; Cell. 2009 Feb 20;136(4):701-18. (PMID: 19239890) ; Genome Res. 2010 Feb;20(2):239-48. (PMID: 20040583) ; J Virol. 1999 Jan;73(1):453-65. (PMID: 9847351) ; Biochem Biophys Res Commun. 2013 Jan 25;430(4):1334-9. (PMID: 23261427) ; Mol Cell Biol. 2007 Dec;27(24):8874-85. (PMID: 17923678) ; Nucleic Acids Res. 2012 Dec;40(22):11380-8. (PMID: 23034805) ; Genes Dev. 2015 Jun 15;29(12 ):1224-38. (PMID: 26080816) ; Cell. 2007 May 18;129(4):823-37. (PMID: 17512414) ; Methods Mol Biol. 2008;435:153-63. (PMID: 18370074) ; Nat Biotechnol. 2010 Jun;28(6):617-623. (PMID: 20473289) ; Genetics. 1993 Mar;133(3):499-508. (PMID: 8384143) ; EMBO J. 1992 Mar;11(3):1145-53. (PMID: 1312461) ; Microbiol Spectr. 2014 Oct;2(5):null. (PMID: 26104370) ; Genetics. 1994 Apr;136(4):1245-59. (PMID: 8013902) ; DNA Repair (Amst). 2009 Apr 5;8(4):461-9. (PMID: 19217833) ; J Virol. 2002 Mar;76(6):2804-16. (PMID: 11861848) ; Genetics. 2004 Nov;168(3):1159-76. (PMID: 15579677) ; Mob DNA. 2012 Aug 02;3(1):12. (PMID: 22856544) ; Nucleic Acids Res. 2014;42(16):10209-25. (PMID: 25147212) ; Mol Cell Biol. 1990 Mar;10 (3):1017-22. (PMID: 2154679) ; Genetics. 2001 Dec;159(4):1449-65. (PMID: 11779788) ; Cell. 2013 Feb 28;152(5):969-83. (PMID: 23452847) ; Mol Cell Biochem. 2001 Nov;227(1-2):59-66. (PMID: 11827175) ; Virus Res. 2008 Jun;134(1-2):221-34. (PMID: 18261821) ; Crit Rev Biochem Mol Biol. 2016;51(1):26-42. (PMID: 26508664) ; Genetics. 2015 Nov;201(3):905-24. (PMID: 26358720) ; Genome Res. 2012 Apr;22(4):704-13. (PMID: 22219511) ; Cell. 1992 May 29;69(5):769-80. (PMID: 1317268) ; Biochem Soc Trans. 2010 Aug;38(4):1110-5. (PMID: 20659013) ; Mol Cell Biol. 2013 Dec;33(24):4779-92. (PMID: 24100010) ; Mol Cell Biol. 1996 Jan;16(1):338-46. (PMID: 8524313) ; Trends Cell Biol. 2002 May;12(5):226-30. (PMID: 12062170) ; Cell Host Microbe. 2013 Sep 11;14(3):232-41. (PMID: 24034610) ; Mol Biol Cell. 2005 Apr;16(4):1823-38. (PMID: 15659641) ; Microbiology. 2016 Jun;162(6):1009-22. (PMID: 27027300) ; Mob DNA. 2015 Dec 08;6:22. (PMID: 26664557) ; Genome Res. 2005 May;15(5):641-54. (PMID: 15837808) ; Nucleic Acids Res. 1995 Nov 11;23(21):4421-5. (PMID: 7501465) ; Chembiochem. 2005 Oct;6(10 ):1735-43. (PMID: 16142820) ; Mol Cell Biol. 2000 Oct;20(20):7798-812. (PMID: 11003674) ; Nat Rev Genet. 2011 May 04;12 (7):459-63. (PMID: 21540878) ; Genome Biol. 2000;1(2):REVIEWS1011. (PMID: 11178233) ; Adv Virus Res. 1999;52:411-26. (PMID: 10384245) ; PLoS One. 2011 Feb 28;6(2):e16719. (PMID: 21386897) ; Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5913-7. (PMID: 8016088) ; J Virol. 1998 Feb;72(2):1324-33. (PMID: 9445033) ; J Biol Chem. 2002 Jul 26;277(30):27489-93. (PMID: 12016221) ; J Biosci Bioeng. 2012 Apr;113(4):526-8. (PMID: 22222142) ; Genetics. 1976 Jun;83(2):285-301. (PMID: 17248715) ; Cold Spring Harb Perspect Med. 2012 Jul;2(7):a006890. (PMID: 22762018) ; Retrovirology. 2008 Jun 13;5:48. (PMID: 18554410) ; Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):936-40. (PMID: 1846969) ; EMBO J. 1993 Dec;12(12):4885-95. (PMID: 8223497) ; Mol Cell Biol. 1999 Aug;19(8):5768-84. (PMID: 10409764) ; Curr Opin Cell Biol. 2014 Jun;28:46-53. (PMID: 24657998) ; Curr Opin Cell Biol. 2011 Feb;23(1):65-70. (PMID: 21030234) ; Cell. 2008 Oct 3;135(1):49-60. (PMID: 18854154) ; Mol Cell Biol. 1998 May;18(5):2502-13. (PMID: 9566871) ; Science. 2008 Feb 15;319(5865):921-6. (PMID: 18187620) ; Genetics. 1991 May;128(1):69-77. (PMID: 1648006) ; Nucleic Acids Res. 2012 Jan;40(Database issue):D695-9. (PMID: 22039153) ; Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1054-63. (PMID: 26858452) ; Cell. 1985 Mar;40(3):491-500. (PMID: 2982495) ; Science. 2015 Sep 25;349(6255):1549-53. (PMID: 26404838) ; Genetics. 2003 Jul;164(3):867-79. (PMID: 12871900) ; J Biol Chem. 2005 Nov 25;280(47):39135-42. (PMID: 16195226) ; Methods Mol Biol. 2006;313:107-20. (PMID: 16118429) ; Viruses. 2013 Oct 07;5(10):2483-511. (PMID: 24103892) ; Cell. 2004 May 14;117(4):427-39. (PMID: 15137937) ; PLoS Genet. 2012;8(10):e1002976. (PMID: 23093942) ; Mol Microbiol. 2015 Aug;97(4):746-58. (PMID: 25983038) ; PLoS One. 2011 Mar 23;6(3):e17862. (PMID: 21448280) ; Yeast. 2006 Feb;23(3):173-83. (PMID: 16498704) ; Microbiol Spectr. 2014 Dec;2(6):null. (PMID: 26104441) ; Cell Host Microbe. 2008 Nov 13;4(5):495-504. (PMID: 18976975) ; J Biol Chem. 1995 Jun 2;270(22):12973-6. (PMID: 7768886) ; Nucleic Acids Res. 2014 Jul;42(13):8449-60. (PMID: 24948612) ; Microbiol Spectr. 2015 Aug;3(4):null. (PMID: 26350316) ; Nature. 2007 Mar 29;446(7135):572-6. (PMID: 17392789) ; Nucleic Acids Res. 2015 Jan;43(Database issue):D656-61. (PMID: 25361970) ; Nature. 1995 Mar 9;374(6518):193-6. (PMID: 7877695) ; EMBO J. 1992 Jan;11(1):291-303. (PMID: 1310932) ; J Virol. 2005 Aug;79(15):9540-55. (PMID: 16014916) ; Cell. 1985 Sep;42(2):507-17. (PMID: 2411424) ; PLoS One. 2011 Mar 23;6(3):e17612. (PMID: 21448273) ; Genes Dev. 2014 May 1;28(9):959-70. (PMID: 24788517) Substance Nomenclature: 0 (Integration Host Factors) ; 0 (Retroelements) ; 0 (Saccharomyces cerevisiae Proteins) ; 0 (Spp382 protein, S cerevisiae) ; EC 2.3.2.27 (Rhp18 protein, S pombe) ; EC 2.3.2.27 (Ubiquitin-Protein Ligases) ; EC 2.7.7.- (Integrases) ; EC 2.7.7.49 (RNA-Directed DNA Polymerase) ; EC 2.7.7.49 (reverse transcriptase Ty3, S cerevisiae) Entry Date(s): Date Created: 20171213 Date Completed: 20180105 Latest Revision: 20181113 Update Code: 20240513 PubMed Central ID: PMC5741268

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Host factors that promote retrotransposon integration are similar in distantly related eukaryotes.