Competing trends of ROS and RNS-mediated protein modifications during hypoxia as an alternate mechanism of NO benefits.
In: Biochimie, Jg. 148 (2018-05-01), S. 127-138
academicJournal
Zugriff:
Hypoxia, especially altitude associated hypoxia is known to cause severe physiological alterations and life-threatening conditions. Impaired redox balance along with oxidative stress, protein carbonylation and instigation of apoptotic events are common sub-cellular events that follow the hypoxic insult. The role of nitric oxide (NO) is very dynamic and versatile in preventing the ill effects of hypoxia vis-a-vis reacting with oxidative species and causing protein nitrosylation. Although several mechanisms of NO-mediated cytoprotection are known during hypoxic insult, limited pieces of evidence are available to support the relationship between two downstream events of oxidative stress, protein carbonylation (caused by carbonyl; CO radical) and protein nitrosylation/nitration (caused by NO/peroxynitrite; ONOO radical). In this study, we investigated an entirely new aspect of NO protection in hypoxia involving crosstalk between carbonylation and nitrosylation. Using standard NO inhibitor l-NAME and simulated hypoxic conditions in hypoxia-sensitive cell line H9c2, we evaluated the levels of radicals, cell death, mitochondrial membrane potential, levels of protein nitrosylation, protein nitration and carbonylation and glutathione content. The results were then carefully analyzed in light of NO bioavailability. Our study shows that reducing NO during hypoxia caused cell death via the increased degree of carbonylation in proteins. This provides a new aspect of NO benefits which furthers opens new possibilities to explore potential mechanisms and effects of cross-talk between nitrosylation, protein nitration and carbonylation, especially through some common antioxidant mediators such as glutathione and thioredoxin.
(Copyright © 2018 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
Titel: |
Competing trends of ROS and RNS-mediated protein modifications during hypoxia as an alternate mechanism of NO benefits.
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Autor/in / Beteiligte Person: | Gangwar, A ; Paul, S ; Ahmad, Y ; Bhargava, K |
Zeitschrift: | Biochimie, Jg. 148 (2018-05-01), S. 127-138 |
Veröffentlichung: | Paris : Editions Scientifiques Elsevier ; <i>Original Publication</i>: Paris., 2018 |
Medientyp: | academicJournal |
ISSN: | 1638-6183 (electronic) |
DOI: | 10.1016/j.biochi.2018.03.009 |
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