Einzeltreffer — DigiBib

Background: The spread of insecticide resistance (IR) is a major threat to vector control programmes for mosquito-borne diseases. Early detection of IR using diagnostic markers could help inform these programmes, especially in remote locations where gathering reliable bioassay data is challenging. Most current molecular tests for genetic IR markers are only suitable for use in well-equipped laboratory settings. There is an unmet need for field-applicable diagnostics.

Methods: A single-cartridge test was designed to detect key IR mutations in the major African vector of malaria, Anopheles gambiae. Developed on the portable, rapid, point-of-care compatible PCR platform - Genedrive® (genedrive® plc), the test comprises two assays which target single nucleotide polymorphisms (SNPs) in the voltage gated sodium channel (VGSC) gene that exert interactive effects on knockdown resistance (kdr): L1014F, L1014S and N1575Y.

Results: Distinct melt peaks were observed for each allele at each locus. Preliminary validation of these assays using a test panel of 70 An. gambiae samples showed complete agreement of our assays with the widely-used TaqMan assays, achieving a sensitivity and specificity of 100%.

Conclusion: Here we show the development of an insecticide resistance detection assay for use on the Genedrive® platform that has the potential to be the first field-applicable diagnostic for kdr.

Titel:	Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae.
Autor/in / Beteiligte Person:	Unwin, VT ; Ainsworth, S ; Rippon, EJ ; Niang, EHA ; Paine, MJI ; Weetman, D ; Adams, ER
Link:	Volltext (PDF) View record at Springer (Volltext)
Zeitschrift:	Parasites & vectors, Jg. 11 (2018-05-18), Heft 1, S. 307
Veröffentlichung:	London : BioMed Central, 2018
Medientyp:	academicJournal
ISSN:	1756-3305 (electronic)
DOI:	10.1186/s13071-018-2893-6
Schlagwort:	Alleles Animals Anopheles drug effects Gene Frequency Genotype Insect Vectors genetics Insecticides pharmacology Pathology, Molecular instrumentation Polymerase Chain Reaction methods Polymorphism, Single Nucleotide genetics Pyrethrins pharmacology Voltage-Gated Sodium Channels genetics Anopheles genetics Genes, Insect drug effects Insecticide Resistance genetics Mutation Pathology, Molecular methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Parasit Vectors] 2018 May 18; Vol. 11 (1), pp. 307. <i>Date of Electronic Publication: </i>2018 May 18. MeSH Terms: Mutation* ; Anopheles / genetics ; Genes, Insect / drug effects ; Insecticide Resistance / genetics ; Pathology, Molecular / methods ; Alleles ; Animals ; Anopheles / drug effects ; Gene Frequency ; Genotype ; Insect Vectors / genetics ; Insecticides / pharmacology ; Pathology, Molecular / instrumentation ; Polymerase Chain Reaction / methods ; Polymorphism, Single Nucleotide / genetics ; Pyrethrins / pharmacology ; Voltage-Gated Sodium Channels / genetics References: PLoS One. 2017 Sep 6;12 (9):e0183084. (PMID: 28877177) ; Trends Parasitol. 2011 Feb;27(2):91-8. (PMID: 20843745) ; Mol Biol Evol. 2010 May;27(5):1117-25. (PMID: 20056691) ; J Clin Microbiol. 2016 Apr;54(4):1051-7. (PMID: 26865685) ; Mol Pharmacol. 2015;87(3):421-9. (PMID: 25523031) ; Malar J. 2007 Aug 13;6:111. (PMID: 17697325) ; Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6614-9. (PMID: 22493253) ; PLoS One. 2016 Nov 16;11(11):e0165925. (PMID: 27851828) ; Trans R Soc Trop Med Hyg. 2009 Nov;103(11):1121-6. (PMID: 19303125) ; Insect Mol Biol. 2005 Dec;14(6):683-8. (PMID: 16313568) ; Infect Genet Evol. 2012 Mar;12(2):487-91. (PMID: 22210132) ; Anal Biochem. 2015 Jun 15;479:28-36. (PMID: 25640587) ; Pest Manag Sci. 2011 Feb;67(2):156-61. (PMID: 20981727) ; Trends Parasitol. 2016 Mar;32(3):187-196. (PMID: 26826784) ; PLoS Negl Trop Dis. 2017 Apr 17;11(4):e0005504. (PMID: 28414744) ; Insect Biochem Mol Biol. 2014 Jul;50:1-17. (PMID: 24704279) ; Malar J. 2006 Feb 22;5:16. (PMID: 16504072) ; Insect Mol Biol. 2007 Jun;16(3):361-75. (PMID: 17433068) ; Trop Med Int Health. 2017 Jun;22(6):755-764. (PMID: 28326655) ; Insect Mol Biol. 1998 May;7(2):179-84. (PMID: 9535162) Grant Information: MC_PC_14111 United Kingdom MRC_ Medical Research Council; MC_PC_15040 United Kingdom MRC_ Medical Research Council; MC PC 14111 United Kingdom MRC_ Medical Research Council; MR/M016447/1 United Kingdom MRC_ Medical Research Council Substance Nomenclature: 0 (Insecticides) ; 0 (Pyrethrins) ; 0 (Voltage-Gated Sodium Channels) Entry Date(s): Date Created: 20180520 Date Completed: 20190130 Latest Revision: 20210109 Update Code: 20240513 PubMed Central ID: PMC5960117

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Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae.