An aryl-homoserine lactone quorum-sensing signal produced by a dimorphic prosthecate bacterium.
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 115 (2018-07-17), Heft 29, S. 7587-7592
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Zugriff:
Many species of Proteobacteria produce acyl-homoserine lactone (AHL) compounds as quorum-sensing (QS) signals for cell density-dependent gene regulation. Most known AHL synthases, LuxI-type enzymes, produce fatty AHLs, and the fatty acid moiety is derived from an acyl-acyl carrier protein (ACP) intermediate in fatty acid biosynthesis. Recently, a class of LuxI homologs has been shown to use CoA-linked aromatic or amino acid substrates for AHL synthesis. By using an informatics approach, we found the CoA class of LuxI homologs exists primarily in α-Proteobacteria. The genome of Prosthecomicrobium hirschii , a dimorphic prosthecate bacterium, possesses a luxI- like AHL synthase gene that we predicted to encode a CoA-utilizing enzyme. We show the P. hirschii LuxI homolog catalyzes synthesis of phenylacetyl-homoserine lactone (PA-HSL). Our experiments show P. hirschii obtains phenylacetate from its environment and uses a CoA ligase to produce the phenylacetyl-CoA substrate for the LuxI homolog. By using an AHL degrading enzyme, we showed that PA-HSL controls aggregation, biofilm formation, and pigment production in P. hirschii These findings advance a limited understanding of the CoA-dependent AHL synthases. We describe how to identify putative members of the class, we describe a signal synthesized by using an environmental aromatic acid, and we identify phenotypes controlled by the aryl-HSL.
Competing Interests: The authors declare no conflict of interest.
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An aryl-homoserine lactone quorum-sensing signal produced by a dimorphic prosthecate bacterium.
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Autor/in / Beteiligte Person: | Liao, L ; Schaefer, AL ; Coutinho, BG ; Brown, PJB ; Greenberg, EP |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 115 (2018-07-17), Heft 29, S. 7587-7592 |
Veröffentlichung: | Washington, DC : National Academy of Sciences, 2018 |
Medientyp: | academicJournal |
ISSN: | 1091-6490 (electronic) |
DOI: | 10.1073/pnas.1808351115 |
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