Negative regulation of adenylate cyclase gene (cya) expression by cyclic AMP-cyclic AMP receptor protein in Escherichia coli: studies with cya-lac protein and operon fusion plasmids.
In: Journal of bacteriology, Jg. 164 (1985-11-01), Heft 2, S. 872-7
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Zugriff:
We constructed cya-lac protein and operon fusion plasmids in vitro. The effect of cyclic AMP (cAMP) on cya expression was examined by measuring the synthesis of beta-galactosidase in Escherichia coli cells containing fused plasmids. In the cya-lacZ fused protein system, cya expression was strongly repressed by exogenous cAMP. Functional cAMP receptor protein (CRP) was necessary for this effect. On the other hand, in a tet-lacZ fused protein as a control system, tet expression was not affected by cAMP. The inhibition of cya expression by cAMP was also observed in the cya-lac fused operon system, although it was necessary to increase the amount of cAMP or CRP in the cells to detect the effect. The results indicate that cAMP-CRP is a negative regulator of cya expression at the level of transcription.
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Negative regulation of adenylate cyclase gene (cya) expression by cyclic AMP-cyclic AMP receptor protein in Escherichia coli: studies with cya-lac protein and operon fusion plasmids.
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Autor/in / Beteiligte Person: | Kawamukai, M ; Kishimoto, J ; Utsumi, R ; Himeno, M ; Komano, T ; Aiba, H |
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Zeitschrift: | Journal of bacteriology, Jg. 164 (1985-11-01), Heft 2, S. 872-7 |
Veröffentlichung: | Washington, DC : American Society for Microbiology, 1985 |
Medientyp: | academicJournal |
ISSN: | 0021-9193 (print) |
DOI: | 10.1128/jb.164.2.872-877.1985 |
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