Metabolite identification of tanshinol borneol ester in rats by high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry.
In: Biomedical chromatography : BMC, Jg. 33 (2019-03-01), Heft 3, S. e4438
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Zugriff:
Tanshinol borneol ester (DBZ) is a potential drug candidate composed of danshensu and borneol. It shows anti-ischemic and anti-atherosclerosis activity. However, little is known about its metabolism in vivo. This research aimed to elucidate the metabolic profile of DBZ through analyzing its metabolites using high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry. Chromatographic separation was performed on an Agilent TC-C 18 column (150 × 4.6 mm, 5.0 μm) with gradient elution using methanol and water containing 0.2% (v/v) formic acid as the mobile phase. Metabolite identification involved analyzing the retention behaviors, changes in molecular weights and MS/MS fragment patterns of DBZ and its metabolites. As a result, 20 potential metabolites were detected and tentatively identified in rat plasma, urine and feces after administration of DBZ. DBZ could be metabolized to O-methylated DBZ, DBZ-O-glucuronide, O-methylated DBZ-O-glucuronide, hydroxylated DBZ and danshensu. Danshensu, a hydrolysis product of DBZ, could further be transformed into 12 metabolites. The proposed method was confirmed to be a reliable and sensitive alternative for characterizing metabolic pathways of DBZ and providing valuable information on its druggability.
(© 2018 John Wiley & Sons, Ltd.)
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Metabolite identification of tanshinol borneol ester in rats by high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry.
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Autor/in / Beteiligte Person: | Wang, J ; Ma, C ; Li, Q ; Wang, X ; Yang, Y ; Yang, L ; Jiang, W ; Liao, S ; Wang, S ; Jia, P ; Zhao, Y ; Zheng, X |
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Zeitschrift: | Biomedical chromatography : BMC, Jg. 33 (2019-03-01), Heft 3, S. e4438 |
Veröffentlichung: | 1990- : Chichester : Wiley ; <i>Original Publication</i>: London : Heyden & Son, c1986-1990, 2019 |
Medientyp: | academicJournal |
ISSN: | 1099-0801 (electronic) |
DOI: | 10.1002/bmc.4438 |
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