DNA polymerase η contributes to genome-wide lagging strand synthesis.
In: Nucleic acids research, Jg. 47 (2019-03-18), Heft 5, S. 2425-2435
Online
academicJournal
Zugriff:
DNA polymerase η (pol η) is best known for its ability to bypass UV-induced thymine-thymine (T-T) dimers and other bulky DNA lesions, but pol η also has other cellular roles. Here, we present evidence that pol η competes with DNA polymerases α and δ for the synthesis of the lagging strand genome-wide, where it also shows a preference for T-T in the DNA template. Moreover, we found that the C-terminus of pol η, which contains a PCNA-Interacting Protein motif is required for pol η to function in lagging strand synthesis. Finally, we provide evidence that a pol η dependent signature is also found to be lagging strand specific in patients with skin cancer. Taken together, these findings provide insight into the physiological role of DNA synthesis by pol η and have implications for our understanding of how our genome is replicated to avoid mutagenesis, genome instability and cancer.
(© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.)
| Titel: |
DNA polymerase η contributes to genome-wide lagging strand synthesis.
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| Autor/in / Beteiligte Person: | Kreisel, K ; Engqvist, MKM ; Kalm, J ; Thompson, LJ ; Boström, M ; Navarrete, C ; McDonald, JP ; Larsson, E ; Woodgate, R ; Clausen, AR |
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| Zeitschrift: | Nucleic acids research, Jg. 47 (2019-03-18), Heft 5, S. 2425-2435 |
| Veröffentlichung: | 1992- : Oxford : Oxford University Press ; <i>Original Publication</i>: London, Information Retrieval ltd., 2019 |
| Medientyp: | academicJournal |
| ISSN: | 1362-4962 (electronic) |
| DOI: | 10.1093/nar/gky1291 |
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