Characterization of a novel cytochrome c <subscript>GJ</subscript> as the electron acceptor of XoxF-MDH in the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV.
In: Biochimica et biophysica acta. Proteins and proteomics, Jg. 1867 (2019-06-01), Heft 6, S. 595-603
academicJournal
Zugriff:
Methanotrophs play a prominent role in the global carbon cycle, by oxidizing the potent greenhouse gas methane to CO 2 . Methane is first converted into methanol by methane monooxygenase. This methanol is subsequently oxidized by either a calcium-dependent MxaF-type or a lanthanide-dependent XoxF-type methanol dehydrogenase (MDH). Electrons from methanol oxidation are shuttled to a cytochrome redox partner, termed cytochrome c L . Here, the cytochrome c L homolog from the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV was characterized. SolV cytochrome c GJ is a fusion of a XoxG cytochrome and a periplasmic binding protein XoxJ. Here we show that XoxGJ functions as the direct electron acceptor of its corresponding XoxF-type MDH and can sustain methanol turnover, when a secondary cytochrome is present as final electron acceptor. SolV cytochrome c GJ (XoxGJ) further displays a unique, red-shifted absorbance spectrum, with a Soret and Q bands at 440, 553 and 595 nm in the reduced state, respectively. VTVH-MCD spectroscopy revealed the presence of a low spin iron heme and the data further shows that the heme group exhibits minimal ruffling. The midpoint potential E m,pH7 of +240 mV is similar to other cytochrome c L type proteins but remarkably, the midpoint potential of cytochrome c GJ was not influenced by lowering the pH. Cytochrome c GJ represents the first example of a cytochrome from a strictly lanthanide-dependent methylotrophic microorganism.
(Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.)
Titel: |
Characterization of a novel cytochrome c <subscript>GJ</subscript> as the electron acceptor of XoxF-MDH in the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV.
|
---|---|
Autor/in / Beteiligte Person: | Versantvoort, W ; Pol, A ; Daumann, LJ ; Larrabee, JA ; Strayer, AH ; Jetten, MSM ; van Niftrik L ; Reimann, J ; Op den Camp, HJM |
Zeitschrift: | Biochimica et biophysica acta. Proteins and proteomics, Jg. 1867 (2019-06-01), Heft 6, S. 595-603 |
Veröffentlichung: | Amsterdam : Elsevier, 2019 |
Medientyp: | academicJournal |
ISSN: | 1878-1454 (electronic) |
DOI: | 10.1016/j.bbapap.2019.04.001 |
Schlagwort: |
|
Sonstiges: |
|