Small, Clickable, and Monovalent Magnetofluorescent Nanoparticles Enable Mechanogenetic Regulation of Receptors in a Crowded Live-Cell Microenvironment.
In: Nano letters, Jg. 19 (2019-06-12), Heft 6, S. 3761-3769
academicJournal
Zugriff:
Multifunctional magnetic nanoparticles have shown great promise as next-generation imaging and perturbation probes for deciphering molecular and cellular processes. As a consequence of multicomponent integration into a single nanosystem, pre-existing nanoprobes are typically large and show limited access to biological targets present in a crowded microenvironment. Here, we apply organic-phase surface PEGylation, click chemistry, and charge-based valency discrimination principles to develop compact, modular, and monovalent magnetofluorescent nanoparticles (MFNs). We show that MFNs exhibit highly efficient labeling to target receptors present in cells with a dense and thick glycocalyx layer. We use these MFNs to interrogate the E-cadherin-mediated adherens junction formation and F-actin polymerization in a three-dimensional space, demonstrating the utility as modular and versatile mechanogenetic probes in the most demanding single-cell perturbation applications.
Titel: |
Small, Clickable, and Monovalent Magnetofluorescent Nanoparticles Enable Mechanogenetic Regulation of Receptors in a Crowded Live-Cell Microenvironment.
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Autor/in / Beteiligte Person: | Kwak, M ; Gu, W ; Jeong, H ; Lee, H ; Lee, JU ; An, M ; Kim, YH ; Lee, JH ; Cheon, J ; Jun, YW |
Zeitschrift: | Nano letters, Jg. 19 (2019-06-12), Heft 6, S. 3761-3769 |
Veröffentlichung: | Washington, DC : American Chemical Society, c2001-, 2019 |
Medientyp: | academicJournal |
ISSN: | 1530-6992 (electronic) |
DOI: | 10.1021/acs.nanolett.9b00891 |
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