Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II <superscript>®</superscript> CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia.
In: Biochemistry and biophysics reports, Jg. 18 (2019-04-28), S. 100635
Online
academicJournal
Zugriff:
This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives were to assess the relationship of the quantity of the free alpha, beta, delta and gamma globin chains with the phenotypic diversity of beta-thalassaemias (β-thal). The results demonstrate that the pools of free globin chains in red blood cells were correlating with the severity of the disease in patients with different phenotypes of β-thal. The mechanism and the regulation of synthesis of free globin chains pool in a normal individual and in patients with different β-thal phenotypes could arise from several mechanisms which will require further investigation. The role of the free globin pool in patients with β-thal for development of novel therapeutic approaches based on these potential targets requires further investigation. Pertinent biomarkers improves the diagnosis of the β-thal, especially in low-income countries where they are most common and allows more effective therapeutic intervention leading to more successful therapeutic outcome.
Titel: |
Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II <superscript>®</superscript> CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia.
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Autor/in / Beteiligte Person: | Abdullah, UYH ; Ibrahim, HM ; Jassim, HM ; Salleh, MZ ; Kek, TL ; Fakhruzzaman Bin Noorizhab, MN ; Zilfalil, BA ; Wilairat, P ; Fucharoen, S |
Link: | |
Zeitschrift: | Biochemistry and biophysics reports, Jg. 18 (2019-04-28), S. 100635 |
Veröffentlichung: | [Amsterdam] : Elsevier B.V., [2015]-, 2019 |
Medientyp: | academicJournal |
ISSN: | 2405-5808 (electronic) |
DOI: | 10.1016/j.bbrep.2019.100635 |
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