Extracellular coenzyme Q <subscript>10</subscript> (CoQ <subscript>10</subscript> ) is reduced to ubiquinol-10 by intact Hep G2 cells independent of intracellular CoQ <subscript>10</subscript> reduction.
In: Archives of biochemistry and biophysics, Jg. 672 (2019-09-15), S. 108067
academicJournal
Zugriff:
Coenzyme Q 10 (CoQ 10 ) is an essential factor in the mitochondrial respiratory chain and is closely associated with ATP production in humans. It is known that orally administered CoQ 10 in humans is rapidly reduced, and most is detected as a reduced form, ubiquinol-10 (CoQ 10 H 2 ), in serum. However, the mechanism of exogenous CoQ 10 reduction in vivo is unclear. Therefore, in order to clarify how CoQ 10 is reduced to CoQ 10 H 2 , we conducted a study using human liver cancer cell line Hep G2 cells, which show strong intracellular CoQ 10 -reducing activity. When intact cells were incubated with CoQ 10 , the exogenously added CoQ 10 was incorporated into the cells, time-, concentration-, and temperature-dependently, and 50-80% of that was detected as CoQ 10 H 2 . On the other hand, a part of the extracellular CoQ 10 was also detected as CoQ 10 H 2 , and the amount was greater than that of the intracellular CoQ 10 H 2 . Furthermore, the CoQ 10 -loaded cells did not leak the intracellular CoQ 10 H 2 (or CoQ 10 ) to the outside of the cells, and modulation of the extracellular CoQ 10 H 2 amount had little effect on the intracellular CoQ 10 or CoQ 10 H 2 contents, suggesting the existence of an individual mechanism of CoQ 10 reduction inside and outside the cells. Moreover, intact cells could reduce CoQ 10 in low-density lipoprotein to CoQ 10 H 2 . Therefore, we concluded that a novel CoQ 10 -reducing mechanism may exist in the plasma membrane, probably the outer surface, of Hep G2 cells, and it may work to reduce extracellular CoQ 10 and/or maintain extracellular CoQ 10 H 2 .
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Titel: |
Extracellular coenzyme Q <subscript>10</subscript> (CoQ <subscript>10</subscript> ) is reduced to ubiquinol-10 by intact Hep G2 cells independent of intracellular CoQ <subscript>10</subscript> reduction.
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Autor/in / Beteiligte Person: | Takahashi, T ; Mine, Y ; Okamoto, T |
Zeitschrift: | Archives of biochemistry and biophysics, Jg. 672 (2019-09-15), S. 108067 |
Veröffentlichung: | <2000- > : San Diego, CA : Elsevier ; <i>Original Publication</i>: New York, NY : Academic Press, 2019 |
Medientyp: | academicJournal |
ISSN: | 1096-0384 (electronic) |
DOI: | 10.1016/j.abb.2019.108067 |
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