Pharmacological characterization, cellular localization and expression profile of NPY receptor subtypes Y2 and Y7 in large yellow croaker, Larimichthys crocea.

Wang, T ; Liang, J ; et al.

In: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Jg. 238 (2019-12-01), S. 110347

academicJournal

Neuropeptide Y (NPY) receptors are suggested to mediate the multi-physiological functions of NPY family peptides, such as food intake, in teleost fish. However, the structure and signaling of fish NPY receptors are yet to be fully elucidated. In this study, we report the cloning and characterization of two neuropeptide Y receptor subtypes, Y2 (NPY2R) and Y7 (NPY7R), in yellow croaker Larimichthys crocea (L. crocea) (LcNPY2R, LcNPY7R). The gene structure, pharmacological characterization, cell location, and tissue expression of these two receptors were explored. The phylogenetic results showed that LcNPY2R and LcNPY7R had typical G protein-coupled receptor profiles, associated with the Y2 subfamily, with coding sequences that are highly conserved in vertebrates. The expression of both LcNPY2R and LcNPY7R could be activated by LcNPY in HEK293 cells. However, truncated LcNPY 18 - 36 was only able to activate LcNPY2R at the same level as full length LcNPY. Expression analysis revealed that LcNPY2R mRNA was predominantly expressed in the intestine and liver, whereas LcNPY7R was expressed in the stomach, which indicated that both receptors were related to the digestive system. Overall, our data establishes a molecular basis to determine the actions of LcNPY2R and LcNPY7R, which could be used to elucidate the conserved roles of these receptor-ligand pairs in vertebrates.

Titel:	Pharmacological characterization, cellular localization and expression profile of NPY receptor subtypes Y2 and Y7 in large yellow croaker, Larimichthys crocea.
Autor/in / Beteiligte Person:	Wang, T ; Liang, J ; Xiang, X ; Chen, X ; Zhang, B ; Zhou, N ; Huang, W ; Yang, J
Zeitschrift:	Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Jg. 238 (2019-12-01), S. 110347
Veröffentlichung:	Oxford : Pergamon ; <i>Original Publication</i>: Oxford : Tarrytown, NY : Pergamon ; Elsevier, c1994-, 2019
Medientyp:	academicJournal
ISSN:	1879-1107 (electronic)
DOI:	10.1016/j.cbpb.2019.110347
Schlagwort:	Animals Gene Expression Profiling HEK293 Cells Humans Organ Specificity physiology Fish Proteins biosynthesis Fish Proteins genetics Gene Expression Regulation physiology Perciformes genetics Perciformes metabolism Receptors, Neuropeptide Y biosynthesis Receptors, Neuropeptide Y genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Comp Biochem Physiol B Biochem Mol Biol] 2019 Dec; Vol. 238, pp. 110347. <i>Date of Electronic Publication: </i>2019 Sep 06. MeSH Terms: Fish Proteins* / biosynthesis ; Fish Proteins* / genetics ; Perciformes* / genetics ; Perciformes* / metabolism ; Receptors, Neuropeptide Y* / biosynthesis ; Receptors, Neuropeptide Y* / genetics ; Gene Expression Regulation / *physiology ; Animals ; Gene Expression Profiling ; HEK293 Cells ; Humans ; Organ Specificity / physiology Contributed Indexing: Keywords: Fish GPCR; Neuropeptide Y receptor; Y2; Y7; large yellow croaker Substance Nomenclature: 0 (Fish Proteins) ; 0 (Receptors, Neuropeptide Y) ; 0 (neuropeptide Y2 receptor) Entry Date(s): Date Created: 20190910 Date Completed: 20191125 Latest Revision: 20191125 Update Code: 20231215

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