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Lignocellulosic biomass provides attractive nonfood carbohydrates for the production of ethanol, and dilute acid pretreatment is a biomass-independent process for access to these carbohydrates. However, this pretreatment also releases volatile and nonvolatile inhibitors of fermenting microorganisms. To identify unique gene products contributing to sensitivity/tolerance to nonvolatile inhibitors, ethanologenic Escherichia coli strain LY180 was adapted for growth in vacuum-treated sugarcane bagasse acid hydrolysate (VBHz) lacking furfural and other volatile inhibitors. A mutant, strain AQ15, obtained after approximately 500 generations of growth in VBHz, grew and fermented the sugars in a medium with 50% VBHz. Comparative genome sequence analysis of strains AQ15 and LY180 revealed 95 mutations in strain AQ15. Six of these mutations were also found in strain SL112, an independent inhibitor-tolerant derivative of strain LY180. Among these six mutations, null mutations in mdh and bacA were identified as contributing factors to VBHz tolerance in strain AQ15, based on the genetic and physiological analysis. The deletion of either gene in strain LY180 increased tolerance to VBHz from approximately 30-50% (vol/vol). Considering the location and physiological role of the two enzymes in the cell, it is likely that the two enzymes contribute to the VBHz sensitivity of ethanologenic E. coli by different mechanisms.

Titel:	Chromosomal mutations in Escherichia coli that improve tolerance to nonvolatile side-products from dilute acid treatment of sugarcane bagasse.
Autor/in / Beteiligte Person:	Shi, A ; Yomano, LP ; York, SW ; Zheng, H ; Shanmugam, KT ; Ingram, LO
Link:	Full Text Finder (Volltext)
Zeitschrift:	Biotechnology and bioengineering, Jg. 117 (2020), Heft 1, S. 85-95
Veröffentlichung:	<2005->: Hoboken, NJ : Wiley ; <i>Original Publication</i>: New York, Wiley., 2020
Medientyp:	academicJournal
ISSN:	1097-0290 (electronic)
DOI:	10.1002/bit.27189
Schlagwort:	Biomass Cellulose chemistry Ethanol chemistry Ethanol metabolism Hydrogen-Ion Concentration Hydrolysis Cellulose metabolism Escherichia coli genetics Escherichia coli metabolism Escherichia coli physiology Mutation genetics Mutation physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Language: English [Biotechnol Bioeng] 2020 Jan; Vol. 117 (1), pp. 85-95. <i>Date of Electronic Publication: </i>2019 Nov 12. MeSH Terms: Escherichia coli* / genetics ; Escherichia coli* / metabolism ; Escherichia coli* / physiology ; Mutation* / genetics ; Mutation* / physiology ; Cellulose / metabolism ; Biomass ; Cellulose / chemistry ; Ethanol / chemistry ; Ethanol / metabolism ; Hydrogen-Ion Concentration ; Hydrolysis References: Alriksson, B., Cavka, A., & Jönsson, L. J. (2011). Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents. Bioresource Technology, 102, 1254-1263. https://doi.org/10.1016/j.biortech.2010.08.037. ; Altaner, C. M., & Saake, B. (2016). 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Grant Information: International BASF Corporation; 2011-10006-30358 International US Department of Agriculture; 2012-67009-19596 International US Department of Agriculture; DE-PI0000031 International US Department of Energy's Office of International Affairs Contributed Indexing: Keywords: bacA; biomass hydrolysate; ethanologenic E. coli; inhibitor tolerance; mdh Substance Nomenclature: 3K9958V90M (Ethanol) ; 9004-34-6 (Cellulose) ; 9006-97-7 (bagasse) Entry Date(s): Date Created: 20191016 Date Completed: 20210201 Latest Revision: 20210201 Update Code: 20240513

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Chromosomal mutations in Escherichia coli that improve tolerance to nonvolatile side-products from dilute acid treatment of sugarcane bagasse.