CE determination of the thermodynamic pK <subscript>a</subscript> values and limiting ionic mobilities of 14 low molecular mass UV absorbing ampholytes for accurate characterization of the pH gradient in carrier ampholytes-based IEF and its numeric simulation.

Ansorge, M ; Gaš, B ; et al.

In: Electrophoresis, Jg. 41 (2020-04-01), Heft 7-8, S. 514-522

Online academicJournal

Zugriff:

Full Text Finder (Volltext)

Fourteen low molecular mass UV absorbing ampholytes containing 1 or 2 weakly acidic and 1 or 2 weakly basic functional groups that best satisfy Rilbe's requirement for being good carrier ampholytes (ΔpK a = pKa monoanion - pKa monocation < 2) were selected from a large group of commercially readily available ampholytes in a computational study using two software packages (ChemSketch and SPARC). Their electrophoretic mobilities were measured in 10 mM ionic strength BGEs covering the 2 < pH < 12 range. Using our Debye-Hückel and Onsager-Fuoss laws-based new software, AnglerFish (freeware, https://echmet.natur.cuni.cz/software/download), the effective mobilities were recalculated to zero ionic strength from which the thermodynamic pK a values and limiting ionic mobilities of the ampholytes were directly calculated by Henderson-Hasselbalch equation-type nonlinear regression. The tabulated thermodynamic pK a values and limiting ionic mobilities of these ampholytes (pI markers) facilitate both the overall and the narrow-segment characterization of the pH gradients obtained in IEF in order to mitigate the errors of analyte ampholyte pI assignments caused by the usual (but rarely proven) assumption of pH gradient linearity. These thermodynamic pK a and limiting mobility values also enable the reality-based numeric simulation of the IEF process using, for example, Simul (freeware, https://echmet.natur.cuni.cz/software/download).

Titel:	CE determination of the thermodynamic pK <subscript>a</subscript> values and limiting ionic mobilities of 14 low molecular mass UV absorbing ampholytes for accurate characterization of the pH gradient in carrier ampholytes-based IEF and its numeric simulation.
Autor/in / Beteiligte Person:	Ansorge, M ; Gaš, B ; Boublík, M ; Malý, M ; Šteflová, J ; Hruška, V ; Vigh, G
Link:	Full Text Finder (Volltext)
Zeitschrift:	Electrophoresis, Jg. 41 (2020-04-01), Heft 7-8, S. 514-522
Veröffentlichung:	<Oct. 2004->: Weinheim : Wiley-VCH ; <i>Original Publication</i>: [Weinheim, Germany] : Verlag Chemie, [1980-, 2020
Medientyp:	academicJournal
ISSN:	1522-2683 (electronic)
DOI:	10.1002/elps.201900381
Schlagwort:	Buffers Computer Simulation Hydrogen-Ion Concentration Osmolar Concentration Thermodynamics Ampholyte Mixtures chemistry Electrophoresis, Capillary methods Isoelectric Focusing methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Electrophoresis] 2020 Apr; Vol. 41 (7-8), pp. 514-522. <i>Date of Electronic Publication: </i>2019 Nov 21. MeSH Terms: Ampholyte Mixtures / chemistry ; Electrophoresis, Capillary / methods ; Isoelectric Focusing / *methods ; Buffers ; Computer Simulation ; Hydrogen-Ion Concentration ; Osmolar Concentration ; Thermodynamics References: Rilbe, H., Ann. N. Y. Acad. Sci. 1973, 209, 11-22. ; Rilbe, H., Electrophoresis 1984, 5, 1-17. ; Frederiksson, S., Anal. Biochem. 1972, 70, 575-585. ; Righetti, P. G., Wenisch, E., Faupel, M., J. Chromaotgr. 1989, 475, 293-307. ; Weber, G., Boček, P., Electrophoresis 1996, 17, 1896-1910. ; Shimura, K., Wang, Z., Matsumoto, H., Kasai, K., Electrophoresis 2000, 21, 603-610. ; Šlais, K., Friedl, Z., J. Chromatogr. A 1994, 661, 249-256. ; Righetti, P. G., Gianazza, E., J. Chromatogr. 1977, 137, 171-181. ; Šlais, K., Friedl, Z., J. Chromatogr. A 1995, 695, 113-122. ; Caslavska, J., Molteni, S., Chmelik, J., Šlais, K., Matulik, F., Thormann, W., J. Chromatogr. A 1995, 680, 549-559. ; Friedl, Z., Šlais, K., Chem. Listy 1997, 91, 679-680. ; Šlais, K., Horká, M., Nováčková, J., Friedl, Z., Electrophoresis 2002, 23, 1682-1688. ; Šťastná, M., Trávníček, M., Šlais, K., Electrophoresis 2005, 26, 53-59. ; Shimura, K., Kamiya, K., Matsumoto, H., Kasai, K., Electrophoresis 2002, 74, 1046-1053. ; Vigh, G., Li, M., US Patent 9,689,841, 2017. ; Klepárník, K., Šlais, K., Boček, P., Electrophoresis 1993, 14, 475-479. ; Lalwani, S., Tutu, E., Vigh, G., Electrophoresis 2005, 26, 2047-2055. ; Šolínová, V., Kašička, B., Electrophoresis 2013, 34, 2655-2665. ; Glukhovskiy, P. V., Vigh, G., Electrophoresis 1998, 19, 3166-3170. ; Hruška, V., Jaroš, M., Gaš, B., Electrophoresis 2006, 27, 984-991. ; Hruška, V., Beneš, M., Svobodová, J., Zusková, I., Gaš, B., Electrophoresis 2012, 33, 938-947. ; Malý, M., Dovhunová, M., Dvořák, M., Gerlor, G. S., Kler, P. A., Hruška, V., Dubský, P., Electrophoresis 2019, 40, 683-692. ; Tahupi, Y., Schmidt, D. E., Lindner, W., Karger, B. L., Anal. Biochem. 1981, 115, 123-129. ; Katayama, H., Ishihama, Y., Asakawa, N., Anal. Chem. 1998, 70, 5272-5277. ; North, R., Vigh, G., Electrophoresis 2008, 29, 1077-1081. ; Lalwani, S., Tutu, E., Vigh, G., Electrophoresis 2005, 26, 2503-2510. ; Cai, J., Smith, J. T., J. High Resolut. Chromatogr. 1992, 15, 30-32. ; Malý, M., Boublík, M., Pocrnić, M., Ansorge, M., Lorinčíková, K., Svobodová, J., Hruška, V., Dubský, P., Gaš, B., Electrophoresis 2020, 41, https://doi.org/10.1002/elps.201900283. ; Debye, P., Hückel, E., Physik. Z. 1923, 24, 185-206. ; Debye, P., Hückel, E., Physik. Z. 1923, 24, 305-325. ; Davies, C. W., Ion Association, Butterworths, London, 1962. ; Onsager, L., Fuoss, R. M., J. Phys. Chem. 1932, 36, 2689-2778. ; Hirokawa, T., Nishimo, M., Aoki, N., Kiso, Y., Sawamoto, Y., Yagi, T., Akiyama, J., J. Chromatogr. 1983, 271, D1-D106. ; Mosher, R. A., Thormann, W., Electrophoresis 1990, 11, 717-723. ; Bossi, A., Righetti, P. G, Electrophoresis 1997, 18, 2012-2018. ; Fitch, C. A., Platzer, G., Okon, M., Garcia-Moreno, B., McIntosh L. P., Protein Sci. 2015, 24, 752-761. Grant Information: Research Gift No. 4135 International Agilent Foundation; Grant No. 18-11776S International Grantová Agentura České Republiky Contributed Indexing: Keywords: Capillary isoelectric focusing; Limiting ionic mobility; Thermodynamic acid dissociation constant; pH Gradient linearity; pI Marker Substance Nomenclature: 0 (Ampholyte Mixtures) ; 0 (Buffers) Entry Date(s): Date Created: 20191114 Date Completed: 20210315 Latest Revision: 20210315 Update Code: 20231215

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.