The chemokine CXCL14 mediates platelet function and migration via direct interaction with CXCR4.
In: Cardiovascular research, Jg. 117 (2021-02-22), Heft 3, S. 903-917
Online
academicJournal
Zugriff:
Aims: Beyond classical roles in thrombosis and haemostasis, it becomes increasingly clear that platelets contribute as key players to inflammatory processes. The involvement of platelets in these processes is often mediated through a variety of platelet-derived chemokines which are released upon activation and act as paracrine and autocrine factors. In this study, we investigate CXCL14, a newly described platelet chemokine and its role in thrombus formation as well as monocyte and platelet migration. In addition, we examine the chemokine receptor CXCR4 as a possible receptor for CXCL14 on platelets. Furthermore, with the use of artificially generated platelets derived from induced pluripotent stem cells (iPSC), we investigate the importance of CXCR4 for CXCL14-mediated platelet functions.
Methods and Results: In this study, we showed that CXCL14 deficient platelets reveal reduced thrombus formation under flow compared with wild-type platelets using a standardized flow chamber. Addition of recombinant CXCL14 normalized platelet-dependent thrombus formation on collagen. Furthermore, we found that CXCL14 is a chemoattractant for platelets and mediates migration via CXCR4. CXCL14 promotes platelet migration of platelets through the receptor CXCR4 as evidenced by murine CXCR4-deficient platelets and human iPSC-derived cultured platelets deficient in CXCR4. We found that CXCL14 directly interacts with the CXCR4 as verified by immunoprecipitation and confocal microscopy.
Conclusions: Our results reveal CXCL14 as a novel platelet-derived chemokine that is involved in thrombus formation and platelet migration. Furthermore, we identified CXCR4 as principal receptor for CXCL14, an interaction promoting platelet migration.
(Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) 2020. For permissions, please email: journals.permissions@oup.com.)
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The chemokine CXCL14 mediates platelet function and migration via direct interaction with CXCR4.
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Autor/in / Beteiligte Person: | Witte, A ; Rohlfing, AK ; Dannenmann, B ; Dicenta, V ; Nasri, M ; Kolb, K ; Sudmann, J ; Castor, T ; Rath, D ; Borst, O ; Skokowa, J ; Gawaz, M |
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Zeitschrift: | Cardiovascular research, Jg. 117 (2021-02-22), Heft 3, S. 903-917 |
Veröffentlichung: | 2008- : Oxford : Oxford Journals ; <i>Original Publication</i>: London, British Medical Assn., 2021 |
Medientyp: | academicJournal |
ISSN: | 1755-3245 (electronic) |
DOI: | 10.1093/cvr/cvaa080 |
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