Einzeltreffer — DigiBib

Current cell-wall models assume no covalent bonding between cellulose and hemicelluloses such as xyloglucan or mixed-linkage β-d-glucan (MLG). However, Equisetum hetero-trans-β-glucanase (HTG) grafts cellulose onto xyloglucan oligosaccharides (XGOs) - and, we now show, xyloglucan polysaccharide - in vitro, thus exhibiting CXE (cellulose:xyloglucan endotransglucosylase) activity. In addition, HTG also catalyzes MLG-to-XGO bonding (MXE activity). In this study, we explored the CXE action of HTG in native plant cell walls and tested whether expansin exposes cellulose to HTG by disrupting hydrogen bonds. To quantify and visualize CXE and MXE action, we assayed the sequential release of HTG products from cell walls pre-labeled with substrate mimics. We demonstrated covalent cellulose-xyloglucan bonding in plant cell walls and showed that CXE and MXE action was up to 15% and 60% of total transglucanase action, respectively, and peaked in aging, strengthening tissues: CXE in xylem and cells bordering intercellular canals and MXE in sclerenchyma. Recombinant bacterial expansin (EXLX1) strongly augmented CXE activity in vitro. CXE and MXE action in living Equisetum structural tissues potentially strengthens stems, while expansin might augment the HTG-catalyzed CXE reaction, thereby allowing efficient CXE action in muro. Our methods will enable surveys for comparable reactions throughout the plant kingdom. Furthermore, engineering similar hetero-polymer formation into angiosperm crop plants may improve certain agronomic traits such as lodging tolerance.

Titel:	Hetero-trans-β-Glucanase Produces Cellulose-Xyloglucan Covalent Bonds in the Cell Walls of Structural Plant Tissues and Is Stimulated by Expansin.
Autor/in / Beteiligte Person:	Herburger, K ; Franková, L ; Pičmanová, M ; Loh, JW ; Valenzuela-Ortega, M ; Meulewaeter, F ; Hudson, AD ; French, CE ; Fry, SC
Link:	Full Text Finder (Volltext)
Zeitschrift:	Molecular plant, Jg. 13 (2020-07-06), Heft 7, S. 1047-1062
Veröffentlichung:	2015- : Cambridge, MA Cell Press ; <i>Original Publication</i>: Oxford, England : Oxford University Press, 2008-, 2020
Medientyp:	academicJournal
ISSN:	1752-9867 (electronic)
DOI:	10.1016/j.molp.2020.04.011
Schlagwort:	Equisetum enzymology Glycosyltransferases metabolism Hydrogen Bonding Cell Wall metabolism Cellulose metabolism Equisetum metabolism Glucans metabolism Glycoside Hydrolases metabolism Plant Proteins metabolism Xylans metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Mol Plant] 2020 Jul 06; Vol. 13 (7), pp. 1047-1062. <i>Date of Electronic Publication: </i>2020 May 04. MeSH Terms: Cell Wall / metabolism ; Cellulose / metabolism ; Equisetum / metabolism ; Glucans / metabolism ; Glycoside Hydrolases / metabolism ; Plant Proteins / metabolism ; Xylans / *metabolism ; Equisetum / enzymology ; Glycosyltransferases / metabolism ; Hydrogen Bonding References: Plant Physiol. 2012 Apr;158(4):1933-43. (PMID: 22362871) ; Biochem J. 1998 Jun 1;332 ( Pt 2):507-15. (PMID: 9601081) ; Plant J. 2008 Jul;55(2):240-52. (PMID: 18397375) ; Planta. 2015 Jan;241(1):83-93. (PMID: 25218793) ; New Phytol. 2008;179(1):104-15. (PMID: 18393951) ; Plant J. 2011 Aug;67(4):662-81. (PMID: 21554451) ; J Exp Bot. 2018 Jan 4;69(2):137-146. (PMID: 29211894) ; Sci Rep. 2017 Apr 26;7:46099. (PMID: 28443615) ; Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:641-664. (PMID: 15012222) ; Am J Bot. 2004 Nov;91(11):1936-8. (PMID: 21652340) ; Plant J. 2018 Jan;93(2):211-226. (PMID: 29160933) ; Plant J. 2015 Sep;83(5):753-69. (PMID: 26185964) ; Plant Physiol Biochem. 2010 Apr;48(4):207-15. (PMID: 20153658) ; Glycobiology. 1999 Jan;9(1):93-100. (PMID: 9884411) ; Plant Physiol. 2018 Jan;176(1):16-27. (PMID: 29138349) ; Ann Bot. 2011 Aug;108(2):307-19. (PMID: 21752793) ; Plant Physiol. 1993 Nov;103(3):987-992. (PMID: 12231995) ; Biochem J. 2017 Mar 8;474(7):1055-1070. (PMID: 28108640) ; Plant J. 1993 Jan;3(1):1-30. (PMID: 8401598) ; Front Plant Sci. 2018 May 03;9:581. (PMID: 29774041) ; Biochem J. 1992 Mar 15;282 ( Pt 3):821-8. (PMID: 1554366) ; Plant J. 2005 May;42(4):525-34. (PMID: 15860011) ; Annu Rev Plant Biol. 2011;62:567-90. (PMID: 21351878) ; Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16444-9. (PMID: 24065828) ; Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) ; Plant Cell. 2012 Nov;24(11):4731-47. (PMID: 23204407) ; J Plant Physiol. 2020 Jun 4;251:153210. (PMID: 32544741) ; New Phytol. 2013 Jan;197(1):111-22. (PMID: 23078260) ; Plant Cell Physiol. 2006 Nov;47(11):1520-9. (PMID: 17012740) ; Plant Mol Biol. 2019 May;100(1-2):181-197. (PMID: 30868545) ; Am J Bot. 1998 Mar;85(3):305. (PMID: 21715290) ; Plant Mol Biol. 2001 Jul;46(5):581-9. (PMID: 11516151) ; Planta. 2008 Apr;227(5):969-80. (PMID: 18057960) ; Biochem J. 1991 Oct 15;279 ( Pt 2):529-35. (PMID: 1953647) ; Ann Bot. 2007 Dec;100(7):1467-73. (PMID: 17916584) ; Plant Biotechnol J. 2015 May;13(4):514-25. (PMID: 25418842) ; Plant Physiol. 2018 Nov;178(3):1207-1221. (PMID: 30224432) ; Plant J. 2010 Nov;64(4):645-56. (PMID: 20822502) ; J Exp Bot. 2016 Jan;67(2):463-76. (PMID: 26608646) ; PLoS One. 2013 Sep 23;8(9):e75022. (PMID: 24086425) ; Plant Cell. 2000 Jul;12(7):1229-37. (PMID: 10899986) ; Bioresour Technol. 2013 Feb;130:629-37. (PMID: 23334020) ; Plant Cell Physiol. 2007 Jun;48(6):843-55. (PMID: 17504814) ; Planta. 1971 Mar;100(1):47-75. (PMID: 24488103) ; J Cell Sci. 2018 Jan 29;131(2):. (PMID: 28827406) ; J Biol Chem. 2007 Apr 27;282(17):12951-62. (PMID: 17329246) ; Microb Biotechnol. 2016 Nov;9(6):727-736. (PMID: 27365165) ; Plant J. 2008 May;54(3):510-21. (PMID: 18284587) ; PLoS One. 2015 Apr 07;10(4):e0123668. (PMID: 25849978) ; Appl Microbiol Biotechnol. 2015 May;99(9):3807-23. (PMID: 25833181) ; New Phytol. 2015 Aug;207(3):893-904. (PMID: 25900772) ; Appl Microbiol Biotechnol. 2013 Jun;97(12):5381-8. (PMID: 23053073) ; Plant J. 2001 Apr;26(1):23-34. (PMID: 11359607) ; PLoS One. 2015 Sep 24;10(9):e0138902. (PMID: 26402242) ; Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):16876-81. (PMID: 18971341) ; Biotechnol Adv. 2016 Sep-Oct;34(5):997-1017. (PMID: 27269671) ; Carbohydr Res. 2007 Jan 15;342(1):44-54. (PMID: 17125755) ; J Exp Bot. 2013 Sep;64(12):3519-50. (PMID: 23956409) ; Plant Cell. 1999 Nov;11(11):2203-16. (PMID: 10559444) ; J Biol Chem. 2011 May 13;286(19):16814-23. (PMID: 21454649) ; Nature. 2000 Sep 21;407(6802):321-6. (PMID: 11014181) Grant Information: BB/N002458/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council Contributed Indexing: Keywords: cell wall; cellulose; hemicelluloses; hetero-trans-β-glucanase; hetero-transglycosylation; xyloglucan Substance Nomenclature: 0 (Glucans) ; 0 (Plant Proteins) ; 0 (Xylans) ; 0 (expansin protein, plant) ; 37294-28-3 (xyloglucan) ; 9004-34-6 (Cellulose) ; EC 2.4.- (Glycosyltransferases) ; EC 2.4.1.207 (xyloglucan - xyloglucosyltransferase) ; EC 3.2.1.- (Glycoside Hydrolases) Entry Date(s): Date Created: 20200508 Date Completed: 20210602 Latest Revision: 20210602 Update Code: 20231215 PubMed Central ID: PMC7339142

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Hetero-trans-β-Glucanase Produces Cellulose-Xyloglucan Covalent Bonds in the Cell Walls of Structural Plant Tissues and Is Stimulated by Expansin.