Einzeltreffer — DigiBib

The pluripotency of embryonic stem cells (ESCs) is controlled by a multilayer regulatory network, of which the key factors include core pluripotency genes Oct4, Sox2 and Nanog , and multiple microRNAs (miRNAs). Recently, long noncoding RNAs (lncRNAs) have been discovered as a class of new regulators for ESCs, and some lncRNAs could function as competing endogenous RNAs (ceRNAs) to regulate mRNAs by competitively binding to miRNAs. Here, we identify mmu-miR-139-5p as a new regulator for Nanog by targeting Nanog 3' untranslated region (UTR) to repress Nanog expression in mouse ESCs and embryos. Such regulation could be released by an ESC-specifically expressed ceRNA named lnc-NAP . The expression of lnc-NAP is activated by OCT4, SOX2, as well as NANOG through promoter binding. Downregulation of lnc-NAP reduces Nanog abundance, which leads to decreased pluripotency of mouse ESCs and embryonic lethality. These results reveal lnc-NAP as a new regulator for Nanog in mouse ESCs, and uncover a feed-forward regulatory loop of Nanog through the participation of lnc-NAP .

Titel:	Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos.
Autor/in / Beteiligte Person:	Xie, D ; Tong, M ; Xia, B ; Feng, G ; Wang, L ; Li, A ; Luo, G ; Wan, H ; Zhang, Z ; Zhang, H ; Yang, YG ; Zhou, Q ; Wang, M ; Wang, XJ
Link:	Full Text Finder (Volltext)
Zeitschrift:	RNA biology, Jg. 18 (2021-06-01), Heft 6, S. 875-887
Veröffentlichung:	2015- : Philadelphia, PA : Taylor & Francis ; <i>Original Publication</i>: Georgetown, TX : Landes Bioscience,, 2021
Medientyp:	academicJournal
ISSN:	1555-8584 (electronic)
DOI:	10.1080/15476286.2020.1827591
Schlagwort:	3' Untranslated Regions genetics Animals Cell Differentiation genetics Embryo, Mammalian cytology Embryo, Mammalian embryology Embryonic Stem Cells cytology Mice, Inbred C57BL Mice, Inbred DBA Mice, Inbred NOD Mice, SCID Nanog Homeobox Protein metabolism Octamer Transcription Factor-3 genetics Octamer Transcription Factor-3 metabolism Promoter Regions, Genetic genetics Protein Binding RNA-Seq methods Reverse Transcriptase Polymerase Chain Reaction methods SOXB1 Transcription Factors genetics SOXB1 Transcription Factors metabolism Mice Embryo, Mammalian metabolism Embryonic Stem Cells metabolism Gene Expression Regulation, Developmental MicroRNAs genetics Nanog Homeobox Protein genetics RNA, Long Noncoding genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [RNA Biol] 2021 Jun; Vol. 18 (6), pp. 875-887. <i>Date of Electronic Publication: </i>2020 Oct 23. MeSH Terms: Gene Expression Regulation, Developmental* ; Embryo, Mammalian / metabolism ; Embryonic Stem Cells / metabolism ; MicroRNAs / genetics ; Nanog Homeobox Protein / genetics ; RNA, Long Noncoding / *genetics ; 3' Untranslated Regions / genetics ; Animals ; Cell Differentiation / genetics ; Embryo, Mammalian / cytology ; Embryo, Mammalian / embryology ; Embryonic Stem Cells / cytology ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Mice, Inbred NOD ; Mice, SCID ; Nanog Homeobox Protein / metabolism ; Octamer Transcription Factor-3 / genetics ; Octamer Transcription Factor-3 / metabolism ; Promoter Regions, Genetic / genetics ; Protein Binding ; RNA-Seq / methods ; Reverse Transcriptase Polymerase Chain Reaction / methods ; SOXB1 Transcription Factors / genetics ; SOXB1 Transcription Factors / metabolism ; Mice References: Nat Methods. 2012 Feb 12;9(4):360-2. (PMID: 22327834) ; Nucleic Acids Res. 2017 Jan 9;45(1):e2. (PMID: 27608726) ; BMC Genomics. 2012 Dec 26;13:727. (PMID: 23268654) ; Science. 1998 Nov 6;282(5391):1145-7. (PMID: 9804556) ; Cell. 2005 Sep 23;122(6):947-56. (PMID: 16153702) ; Cell. 2009 May 15;137(4):647-58. (PMID: 19409607) ; Biochem Biophys Res Commun. 2016 May 27;474(2):296-302. (PMID: 27105918) ; BMC Bioinformatics. 2009 Aug 04;10:239. (PMID: 19653905) ; Genomics Proteomics Bioinformatics. 2016 Apr;14(2):73-80. (PMID: 27163185) ; FEBS J. 2016 Apr;283(8):1438-52. (PMID: 26859226) ; Int J Biochem Cell Biol. 2012 Sep;44(9):1465-72. (PMID: 22642900) ; Cell. 2011 Mar 18;144(6):940-54. (PMID: 21414485) ; Nature. 2012 Feb 12;483(7390):470-3. (PMID: 22327294) ; Cell. 2008 Feb 22;132(4):567-82. (PMID: 18295576) ; Genome Biol. 2009;10(3):R25. (PMID: 19261174) ; J Genet Genomics. 2017 Apr 20;44(4):179-189. (PMID: 28411033) ; Mol Ther Nucleic Acids. 2012 Aug 07;1:e34. (PMID: 23344176) ; J Biol Chem. 2010 Jun 18;285(25):19483-90. (PMID: 20382743) ; Nat Biotechnol. 2010 May;28(5):511-5. (PMID: 20436464) ; Nature. 2008 Oct 23;455(7216):1124-8. (PMID: 18806776) ; Nature. 1981 Jul 9;292(5819):154-6. (PMID: 7242681) ; Nat Commun. 2016 Apr 12;7:11268. (PMID: 27068353) ; Bioinformatics. 2009 May 1;25(9):1105-11. (PMID: 19289445) ; Nat Cell Biol. 2006 Oct;8(10):1114-23. (PMID: 16980957) ; Genome Biol. 2008;9(9):R137. (PMID: 18798982) ; Cell. 2003 May 30;113(5):631-42. (PMID: 12787504) ; J Biol Chem. 2005 Jul 1;280(26):24731-7. (PMID: 15860457) ; Nature. 2012 Aug 2;488(7409):116-20. (PMID: 22763441) ; Nat Genet. 2006 Apr;38(4):431-40. (PMID: 16518401) ; Biochem Biophys Res Commun. 2009 Dec 25;390(4):1278-82. (PMID: 19883627) ; Nucleic Acids Res. 2016 Dec 15;44(22):10603-10618. (PMID: 27625395) ; Cell. 2008 Jun 13;133(6):1106-17. (PMID: 18555785) ; Dev Cell. 2013 Apr 15;25(1):69-80. (PMID: 23541921) ; Nat Biotechnol. 2011 Jan;29(1):24-6. (PMID: 21221095) ; Nucleic Acids Res. 2017 Jul 3;45(W1):W12-W16. (PMID: 28521017) ; Mech Dev. 2005 Jan;122(1):67-79. (PMID: 15582778) ; Nature. 2007 Dec 20;450(7173):1230-4. (PMID: 18097409) ; Cell. 2008 Aug 8;134(3):521-33. (PMID: 18692474) ; Nucleic Acids Res. 2013 Apr 1;41(6):e74. (PMID: 23335781) ; Blood. 2016 Oct 27;128(17):2117-2129. (PMID: 27605510) ; Mol Cancer. 2014 Apr 28;13:92. (PMID: 24775712) ; Nat Cell Biol. 2012 Nov;14(11):1114-21. (PMID: 23131918) ; Stem Cells. 2008 Jan;26(1):17-29. (PMID: 17916804) ; Nature. 2011 Aug 28;477(7364):295-300. (PMID: 21874018) ; Nature. 2009 Sep 3;461(7260):86-90. (PMID: 19672241) ; Mol Cell. 2014 Mar 20;53(6):1005-19. (PMID: 24530304) Contributed Indexing: Keywords: Nanog; ceRNA; lncRNA; miR-139-5p; microRNA; pluripotency Substance Nomenclature: 0 (3' Untranslated Regions) ; 0 (MIRN139 microRNA, mouse) ; 0 (MicroRNAs) ; 0 (Nanog Homeobox Protein) ; 0 (Nanog protein, mouse) ; 0 (Octamer Transcription Factor-3) ; 0 (Pou5f1 protein, mouse) ; 0 (RNA, Long Noncoding) ; 0 (SOXB1 Transcription Factors) ; 0 (Sox2 protein, mouse) Entry Date(s): Date Created: 20200929 Date Completed: 20211122 Latest Revision: 20240226 Update Code: 20240226 PubMed Central ID: PMC8081037

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Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos.