Hepatitis E Virus Capsid Antigen (HEV-Ag) - A practical diagnostic biomarker in the HEV outbreak scenario.

Lytton, SD ; Bulbul, MRH ; et al.

In: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, Jg. 134 (2021), S. 104692

academicJournal

Wie komme ich dran?

Background: The increased global incidence of hepatitis E virus (HEV) infections, warrants accurate and affordable diagnostics across different geographical regions. The soluble and highly conserved HEV open reading frame 2 (ORF2) capsid antigen (HEV-Ag) is detectable in self-limited acute enteric hepatitis by HEV-Ag ELISA which is a promising serological assay in settings where HEV-RNA testing is not feasible. Our aim was to assess the HEV-Ag biomarker in an HEV outbreak in a low income country.

Methods: A prospective single center longitudinal study during HEV outbreaks in the Chittagong, Bangladesh region between October 2018 and October 2019 was conducted based on recruitment of acute jaundice cases with clinical signs and symptoms of suspect HEV infections. Acute HEV infection was defined as a positive test result for anti-HEV IgM antibodies.

Results: Forty four of the 51 enrolled enteric hepatitis cases (86 %) were confirmed HEV by anti-HEV IgM ELISA at day 0 hospital entry. The anti-HEV-IgM and IgG were positive in all patients and did not reveal significant differences; neither between the time points day 0 and follow-up hospitalization on day 2-6 or day 7-10 nor between RNA-positive (n = 36) versus RNAnegative (n = 8) HEV groups. The HEV-Ag positivity was higher in viral RNA-positive (29/36, 81 %) than the viral RNA-negative (1/8, 12 %) group, p < 0.001 and the HEV-Ag levels positively correlated with viremia, r = 0.77, p < 0.0001. All non-HEV cases; n = 7 tested negative anti-HEV IgM and HEV-Ag and 5 of 7 (71 %) tested anti-HAV IgM positive.

Conclusions: The HEV-Ag ELISA is a reliable and practical diagnostic tool in this acute HEV outbreak.

Titel:	Hepatitis E Virus Capsid Antigen (HEV-Ag) - A practical diagnostic biomarker in the HEV outbreak scenario.
Autor/in / Beteiligte Person:	Lytton, SD ; Bulbul, MRH ; Barua, K ; Begum, MC ; Chowdhury, B ; Islam, Z ; Faiyaz, KI ; Khan Chandan, MS ; Shakeel, A ; Landt, O ; Mamunur, R
Zeitschrift:	Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, Jg. 134 (2021), S. 104692
Veröffentlichung:	Amsterdam : Elsevier Science, c1998-, 2021
Medientyp:	academicJournal
ISSN:	1873-5967 (electronic)
DOI:	10.1016/j.jcv.2020.104692
Schlagwort:	Bangladesh Biomarkers Capsid Disease Outbreaks Hepatitis Antibodies Humans Immunoglobulin M Longitudinal Studies Prospective Studies Hepatitis E diagnosis Hepatitis E epidemiology Hepatitis E virus
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Clin Virol] 2021 Jan; Vol. 134, pp. 104692. <i>Date of Electronic Publication: </i>2020 Nov 16. MeSH Terms: Hepatitis E* / diagnosis ; Hepatitis E* / epidemiology ; Hepatitis E virus* ; Bangladesh ; Biomarkers ; Capsid ; Disease Outbreaks ; Hepatitis Antibodies ; Humans ; Immunoglobulin M ; Longitudinal Studies ; Prospective Studies Contributed Indexing: Keywords: Anti-HEV IgG; Anti-HEV IgM; HEV-Ag ELISA Wantai; Hepatitis E virus capsid antigen Substance Nomenclature: 0 (Biomarkers) ; 0 (Hepatitis Antibodies) ; 0 (Immunoglobulin M) Entry Date(s): Date Created: 20201214 Date Completed: 20210917 Latest Revision: 20210917 Update Code: 20231215

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.