The N-terminal Leu-Pro-Gln sequence of Rab34 is required for ciliogenesis in hTERT-RPE1 cells.
In: Small GTPases, Jg. 13 (2022), Heft 1, S. 77-83
Online
academicJournal
Zugriff:
We have previously shown that Rab34 is an important regulator of ciliogenesis and that its unique long N-terminal region (amino acids 1-49) is essential for ciliogenesis in certain cultured mammalian cells. In the present study, we performed an in-depth deletion analysis of the N-terminal region of Rab34 together with Ala-based site-directed mutagenesis to identify the essential amino acids that are required for serum-starvation-induced ciliogenesis in hTERT-RPE1 cells. The results showed that a Rab34 mutant lacking an N-terminal 18 amino acids and a Rab34 mutant carrying an LPQ-to-AAA mutation (amino acids 16-18) failed to rescue a Rab34-KO phenotype (i.e., defect in ciliogenesis). Our findings suggest that the LPQ sequence of Rab34 is crucial for ciliogenesis in hTERT-RPE1 cells. Abbreviations: AA, amino acid(s); ac-Tub, acetylated tubulin; bsr, blasticidin S-resistant gene; HRP, horseradish peroxidase; hTERT-RPE1, human telomerase reverse transcriptase retinal pigment epithelium 1; KO, knockout; NS, not significant; PBS, phosphate-buffered saline; puro, puromycin-resistant gene.
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The N-terminal Leu-Pro-Gln sequence of Rab34 is required for ciliogenesis in hTERT-RPE1 cells.
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Autor/in / Beteiligte Person: | Oguchi, ME ; Homma, Y ; Fukuda, M |
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Zeitschrift: | Small GTPases, Jg. 13 (2022), Heft 1, S. 77-83 |
Veröffentlichung: | 2015- : Philadelphia, PA : Taylor & Francis ; <i>Original Publication</i>: Austin, TX : Landes Bioscience, 2022 |
Medientyp: | academicJournal |
ISSN: | 2154-1256 (electronic) |
DOI: | 10.1080/21541248.2021.1894910 |
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