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The determination of psychotropic drugs and metabolites in blood is relevant in the context of both therapeutic drug monitoring and clinical and forensic toxicology. LC-MS/MS is the preferred method for these assays. However, LC-MS/MS is particularly susceptible to matrix ionization effects and appropriate sample preparation is required to minimize these effects. In this study, a simple, single-step, mini-QuEchERS extraction procedure, coupled to UPLC-MS/MS, was developed and validated for the determination of 15 toxicologically relevant compounds in whole blood, including psychoactive drugs and some metabolites. The assay was linear in the range of 25-1,000 ng ml -1 , fulfilling criteria for accuracy and precision. Extraction yields (71.9-87.7%) and matrix effects (-3.3 to +4.4%, with the exception of codeine, which had matrix effects of -35.36 to -28.14%) were acceptable for the majority of the evaluated compounds, using a single internal standard. The assay was applied to 238 clinical specimens from patients admitted to an emergency service, with 22 samples presenting quantifiable concentrations of 11 different compounds. The developed assay is a simple and efficient strategy for determination of target psychotropic drugs and metabolites in forensic and clinical toxicology.

Titel:	Simple extraction of toxicologically relevant psychotropic compounds and metabolites from whole blood using mini-QuEChERS followed by UPLC-MS/MS analysis.
Autor/in / Beteiligte Person:	da Silva CP ; Dal Piaz, LPP ; Gerbase, FE ; Müller, VV ; Lizot, LLF ; Antunes, MV ; Linden, R
Link:	Full Text Finder (Volltext)
Zeitschrift:	Biomedical chromatography : BMC, Jg. 35 (2021-09-01), Heft 9, S. e5142
Veröffentlichung:	1990- : Chichester : Wiley ; <i>Original Publication</i>: London : Heyden & Son, c1986-1990, 2021
Medientyp:	academicJournal
ISSN:	1099-0801 (electronic)
DOI:	10.1002/bmc.5142
Schlagwort:	Adolescent Adult Aged Aged, 80 and over Female Humans Linear Models Male Middle Aged Reproducibility of Results Sensitivity and Specificity Young Adult Chromatography, High Pressure Liquid methods Psychotropic Drugs blood Psychotropic Drugs isolation & purification Psychotropic Drugs metabolism Tandem Mass Spectrometry methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biomed Chromatogr] 2021 Sep; Vol. 35 (9), pp. e5142. <i>Date of Electronic Publication: </i>2021 Apr 26. MeSH Terms: Psychotropic Drugs* / blood ; Psychotropic Drugs* / isolation & purification ; Psychotropic Drugs* / metabolism ; Chromatography, High Pressure Liquid / methods ; Tandem Mass Spectrometry / methods ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Linear Models ; Male ; Middle Aged ; Reproducibility of Results ; Sensitivity and Specificity ; Young Adult References: Anastassiades, M., Lehotay, S. J., Stajnbaher, D., & Schenck, F. (2003). Fast and easy multiresidue method employing acetonitrile. Journal of AOAC International, 86(2), 412-431. https://doi.org/10.1093/jaoac/86.2.412. ; Anzillotti, L., Odoardi, S., & Strano-Rossi, S. (2014). 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Legal Medicine, 14(6), 286-296. https://doi.org/10.1016/j.legalmed.2012.04.008. ; Usui, K., Hayashizaki, Y., Minagawa, T., Hashiyada, M., Nakano, A., & Funayama, M. (2012). Rapid determination of disulfoton and its oxidative metabolites in human whole blood and urine using QuEChERS extraction and liquid chromatography-tandem mass spectrometry. Legal Medicine, 14(6), 309-316. https://doi.org/10.1016/j.legalmed.2012.06.005. Grant Information: CNPQ 465450/2014-8 National Institute of Science and Technology in Forensic Sciences; National Council for Scientific and Technological Development; Ministry of Education, Brazil Contributed Indexing: Keywords: UPLC-MS/MS; mini-QuEchERS; psychotropic drugs; whole blood Substance Nomenclature: 0 (Psychotropic Drugs) Entry Date(s): Date Created: 20210417 Date Completed: 20210928 Latest Revision: 20210928 Update Code: 20231215

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Simple extraction of toxicologically relevant psychotropic compounds and metabolites from whole blood using mini-QuEChERS followed by UPLC-MS/MS analysis.