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Granulosa cells (GCs) play an important role in the development of follicles. In this study, we investigate the impact of heat stress at 41°C and 43°C on duck GCs' proliferation and steroids secretion. And, the transcriptomic responses to heat treatment were examined using RNA-sequencing analysis. Digital gene expression profiling was used to screen and identify differentially expressed genes (fold change ≥ 2 and Q value < 0.05). Further, the differential expression genes (DEGs) were classified into GO categories and KEGG pathways. The results show that duck GCs blocked in the G1 phase were increased on exposure to heat stress. Meanwhile, the expression of proliferative genes, which were essential for the transition from G1 to S phase, was inhibited. At the same time, heat stress inhibited the estradiol synthesis of GCs by decreasing CYP11A1 and CYP19A1 gene expression. A total of 241 DEGs including 181 upregulated and 60 downregulated ones were identified. Transcriptome result shows that heat shock protein and CXC chemokines gene were significantly activated during heat stress. While collagenases (MMP1 and MMP13) and strome lysins (MMP3) were downregulated. And, the hedgehog signaling pathway may be a prosurvival adaptive response under heat stress. These results offer a basis for better understanding the molecular mechanism underlying lay-eggs-less in ducks under heat stress.

Titel:	The effect of heat stress on proliferation, synthesis of steroids, and gene expression of duck granulosa cells.
Autor/in / Beteiligte Person:	Yang, C ; Huang, XB ; Chen, SJ ; Li, XJ ; Fu, XL ; Xu, DN ; Tian, YB ; Liu, WJ ; Huang, YM
Link:	Volltext (PDF)
Zeitschrift:	Animal science journal = Nihon chikusan Gakkaiho, Jg. 92 (2021), Heft 1, S. e13617
Veröffentlichung:	Richmond, Vic. : Wiley ; <i>Original Publication</i>: Tokyo, Japan : Japanese Society of Zootechnical Science [1999-, 2021
Medientyp:	academicJournal
ISSN:	1740-0929 (electronic)
DOI:	10.1111/asj.13617
Schlagwort:	Animals Aromatase genetics Aromatase metabolism Chemokines, CXC genetics Chemokines, CXC metabolism Cholesterol Side-Chain Cleavage Enzyme genetics Cholesterol Side-Chain Cleavage Enzyme metabolism Down-Regulation Female Granulosa Cells metabolism Heat-Shock Proteins metabolism Hedgehog Proteins metabolism Matrix Metalloproteinase 1 metabolism Matrix Metalloproteinase 3 metabolism Signal Transduction genetics Signal Transduction physiology Cell Proliferation genetics Ducks physiology Estradiol metabolism Gene Expression Gene Expression Regulation, Developmental genetics Granulosa Cells physiology Heat-Shock Response genetics Heat-Shock Response physiology Ovulation physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Anim Sci J] 2021 Jan-Dec; Vol. 92 (1), pp. e13617. MeSH Terms: Gene Expression* ; Cell Proliferation / genetics ; Ducks / physiology ; Estradiol / metabolism ; Gene Expression Regulation, Developmental / genetics ; Granulosa Cells / physiology ; Heat-Shock Response / genetics ; Heat-Shock Response / physiology ; Ovulation / physiology ; Animals ; Aromatase / genetics ; Aromatase / metabolism ; Chemokines, CXC / genetics ; Chemokines, CXC / metabolism ; Cholesterol Side-Chain Cleavage Enzyme / genetics ; Cholesterol Side-Chain Cleavage Enzyme / metabolism ; Down-Regulation ; Female ; Granulosa Cells / metabolism ; Heat-Shock Proteins / metabolism ; Hedgehog Proteins / metabolism ; Matrix Metalloproteinase 1 / metabolism ; Matrix Metalloproteinase 3 / metabolism ; Signal Transduction / genetics ; Signal Transduction / physiology References: Alemu, T. W., Pandey, H. O., Salilew, W. 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Grant Information: 2018YFE0128200 National Key Technologies R&D Program of China; 2016YFD0500510 National Key Technologies R&D Program of China; 2018A030313630 Natural Science Foundation of Guangdong Province; 2020KJ137 Modern Agriculture Waterfowl Industry Technology System Innovation Team of Guangdong Contributed Indexing: Keywords: duck; granulosa cells; heat stress; transcriptome Substance Nomenclature: 0 (Chemokines, CXC) ; 0 (Heat-Shock Proteins) ; 0 (Hedgehog Proteins) ; 4TI98Z838E (Estradiol) ; EC 1.14.14.1 (Aromatase) ; EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme) ; EC 3.4.24.17 (Matrix Metalloproteinase 3) ; EC 3.4.24.7 (Matrix Metalloproteinase 1) Entry Date(s): Date Created: 20210818 Date Completed: 20210910 Latest Revision: 20230503 Update Code: 20231215

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The effect of heat stress on proliferation, synthesis of steroids, and gene expression of duck granulosa cells.